研究領域 腫瘤  免疫學  生長因子和激素  

抗體來源 Rabbit

克隆類型 Polyclonal

交叉反應 Human, Mouse,  (predicted: Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep, Guinea Pig, )

產(chǎn)品應用 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1ug/Test IF=1:100-500 （石蠟切片需做抗原修復）

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

分 子 量   54/80kDa

細胞定位 分泌型蛋白 

性    狀     Liquid

濃    度    1mg/ml

免 疫 原   KLH conjugated synthetic peptide derived from human HGF alpha:201-300/723 

亞    型IgG

純化方法affinity purified by Protein A

儲 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

保存條件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

PubMedPubMed

產(chǎn)品介紹Hepatocyte growth factor regulates cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor. Hepatocyte growth factor is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability to stimulate mitogenesis, cell motility, and matrix invasion gives it a central role in angiogenesis, tumorogenesis, and tissue regeneration. It is secreted as a single inactive polypeptide and is cleaved by serine proteases into a 69-kDa alpha-chain and 34-kDa beta-chain. A disulfide bond between the alpha and beta chains produces the active, heterodimeric molecule. The protein belongs to the lasminogen subfamily of S1 peptidases but has no detectable protease activity. Alternative splicing of this gene produces multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008].

Function:

HGF is a potent mitogen for mature parenchymal hepatocyte cells, seems to be an hepatotrophic factor, and acts as growth factor for a broad spectrum of tissues and cell types. It has no detectable protease activity.

Subunit:

Dimer of an alpha chain and a beta chain linked by a disulfide bond.

DISEASE:

Defects in HGF are the cause of deafness autosomal recessive type 39 (DFNB39) [MIM:608265]. A form of profound prelingual sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.

Similarity:

Belongs to the peptidase S1 family. Plasminogen subfamily.

Contains 4 kringle domains.

Contains 1 PAN domain.

Contains 1 peptidase S1 domain.

SWISS:

P14210

Gene ID:

3082

Important Note:

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

肝細胞生長因子（HGF)是一種多肽生長因子，具有促進包括肝細胞、上皮細胞、內(nèi)皮細胞、造血細胞等多種類型細胞的生長、遷移和形態(tài)發(fā)生的作用. 它還參與多種細胞的增殖、遷移,對各類腫瘤的侵襲轉移有著重要的誘導作用.

HGF除了由肝臟大量產(chǎn)生并釋放進入血液，還可從胚胎肺成纖維細胞株中分離得到，因此又稱擴散因子（scatter factor）或稱肺成纖維細胞衍生生長因子（fibroblast-derived growth factor）。產(chǎn)品圖片