| 英文名稱 | Serum albumin(Serum Loading Control) |
| 中文名稱 | 人血清白蛋白（內(nèi)參）單克隆抗體 |
| 別 名 | ALB; ALBU_HUMAN; Albumin (32 AA); Albumin (AA 34); Analbuminemia; Bisalbuminemia; Cell growth inhibiting protein 42; DKFZp779N1935; Dysalbuminemic hyperthyroxinemia; Growth inhibiting protein 20; HSA; Hyperthyroxinemia dysalbuminemic; PRO0883; PRO0903; PRO1341; PRO2044; PRO2619; Serum albumin. |
| 產(chǎn)品類型 | 內(nèi)參抗體 |
| 研究領域 | 細胞生物 免疫學 |
| 抗體來源 | Mouse |
| 克隆類型 | Monoclonal |
| 克 隆 號 | 3F4 |
| 交叉反應 | Human, Mouse, (predicted: Rat, Pig, ) |
| 產(chǎn)品應用 | WB=1:500-2000 ELISA=1:1000-15000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 69kDa |
| 細胞定位 | 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | human Serum albumin : |
| 亞 型 | IgG1 |
| 純化方法 | affinity purified by Protein G |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 產(chǎn)品介紹 | Albumin is a soluble, monomeric protein which comprises about one-half of the blood serum protein. Albumin functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Mutations in this gene on chromosome 4 result in various anomalous proteins. Albumin is a globular unglycosylated serum protein of molecular weight 65,000. The human albumin gene is 16,961 nucleotides long from the putative 'cap' site to the first poly(A) addition site. It is split into 15 exons which are symmetrically placed within the 3 domains that are thought to have arisen by triplication of a single primordial domain. Albumin is synthesized in the liver as preproalbumin which has an N-terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin. Function: Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc. Subcellular Location: Secreted. Tissue Specificity: Plasma. Post-translational modifications: Kenitra variant is partially O-glycosylated at Thr-620. It has two new disulfide bonds Cys-600 to Cys-602 and Cys-601 to Cys-606. Glycated in diabetic patients. Phosphorylation sites are present in the extracelllular medium. Acetylated on Lys-223 by acetylsalicylic acid. DISEASE: Defects in ALB are a cause of familial dysalbuminemic hyperthyroxinemia (FDH) [MIM:103600]. FDH is a form of euthyroid hyperthyroxinemia that is due to increased affinity of ALB for T(4). It is the most common cause of inherited euthyroid hyperthyroxinemia in Caucasian population. Similarity: Belongs to the ALB/AFP/VDB family. Contains 3 albumin domains. SWISS: P02769 Gene ID: 280717 Database links: Entrez Gene: 213 Human Omim: 103600 Human SwissProt: P02768 Human Unigene: 418167 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 產(chǎn)品圖片 |  Sample: Lung(Mouse)Lysate at 40 ug Spleen(Mouse)Lysate at 40 ug Primary: Anti-Serum albumin(0945M)at 1/300 dilution Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution Predicted band size: 69kD Observed band size: 69kD  Sample: Lane 1: Serum (Human) Lysate at 20 ug Primary: Anti-Serum albumin (0945M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 67 kD Observed band size: 67 kD  Sample:A549 (Human)Cell Lysate at 40 ug Primary: Anti-Serum albumin(0945M)at 1/300 dilution Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution Predicted band size: 69kD Observed band size: 69kD  Tissue/cell: human hepatocellular carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-ALB Monoclonal Antibody, Unconjugated(bsm-0945M) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0024) and DAB(C-0010) staining |
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