| 產(chǎn)品編號 | Ys-1164R |
| 英文名稱 | Perilipin-2 |
| 中文名稱 | 脂肪分化相關(guān)蛋白抗體 |
| 別 名 | ADFP; Adipophilin; Adipose differentiation related protein; Adipose differentiation-related protein; PLIN2; PLIN2_HUMAN; adipophilin; Adipose differentiation related protein; ADRP; MGC10598 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human, Mouse, (predicted: Rat, ) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復(fù)） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 48kDa |
| 細(xì)胞定位 | 細(xì)胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ADFP: 31-130/422 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 | The protein encoded by this gene belongs to the perilipin family, members of which coat intracellular lipid storage droplets. This protein is associated with the lipid globule surface membrane material, and maybe involved in development and maintenance of adipose tissue. However, it is not restricted to adipocytes as previously thought, but is found in a wide range of cultured cell lines, including fibroblasts, endothelial and epithelial cells, and tissues, such as lactating mammary gland, adrenal cortex, Sertoli and Leydig cells, and hepatocytes in alcoholic liver cirrhosis, suggesting that it may serve as a marker of lipid accumulation in diverse cell types and diseases. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Mar 2011] Function: May be involved in development and maintenance of adipose tissue. Subcellular Location: Membrane; Peripheral membrane protein. Tissue Specificity: Milk lipid globules. Post-translational modifications: Acylated; primarily with C14, C16 and C18 fatty acids. Similarity: Belongs to the perilipin family. SWISS: Q99541 Gene ID: 123 Adipophilin（ADFP）是一種脂滴周圍主要的相關(guān)蛋白，它大量分布在脂質(zhì)蓄積正�；虿徽５募�(xì)胞中，是脂質(zhì)蓄積的一個(gè)特異性標(biāo)記物。它在脂肪細(xì)胞分化早期就有很高的表達(dá)，但當(dāng)脂肪細(xì)胞成熟后，它的表達(dá)就明顯減少，故稱；脂肪分化相關(guān)蛋白。 ADFP在很多組織器官中都發(fā)揮重要作用，它不僅參與脂肪細(xì)胞的脂質(zhì)代謝、脂滴的形成及肝內(nèi)三酰甘油（TO）的合成與代謝，還能促進(jìn)巨噬細(xì)胞、平滑肌細(xì)胞的泡沫化，長鏈脂肪酸的攝取，乳汁的分泌等。 ADFP的表達(dá)異常與動(dòng)脈粥樣硬化、胰島素抵抗和腫瘤等病理過程密切相關(guān)。 |
| 產(chǎn)品圖片 |  Sample: Liver (Mouse) Lysate at 40 ug Primary: Anti-ADFP (bs-1164R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 48 kD Observed band size: 48 kD Tissue/cell: human placenta tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-ADFP Polyclonal Antibody, Unconjugated(bs-1164R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |
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