產品編號 | Ys-1374R |
英文名稱 | BMP4 |
中文名稱 | 骨形態(tài)發(fā)生蛋白4抗體 |
別 名 | BMP 4; BMP2B; BMP2B1; Bone morphogenetic protein 4; DVR4; ZYME; BMP4_HUMAN; BMP-4; Bone morphogenetic protein 2B; BMP-2B. |
抗體來源 | Rabbit |
克隆類型 | Polyclonal |
交叉反應 | Mouse, Sheep, (predicted: Human, Rat, ) |
產品應用 | WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 (石蠟切片需做抗原修復) not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理論分子量 | 13/45kDa |
細胞定位 | 細胞外基質 分泌型蛋白 |
性 狀 | Liquid |
濃 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human BMP4: 251-350/408 |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
緩 沖 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
骨形態(tài)發(fā)生蛋白-4(BMP4)是轉化生長因子β超家族成員,也參與器官發(fā)生,細胞增殖、分化及凋亡等。 | |
產品圖片 | Sample: Sheep skin (Sheep) Lysate at 40 ug Primary: Anti-BMP4 (bs-1374R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 13/45 kD Observed band size: 50 kD Paraformaldehyde-fixed, paraffin embedded (mouse pancreas tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (BMP4) Polyclonal Antibody, Unconjugated (bs-1374R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. |
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