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骨形態(tài)發(fā)生蛋白4抗體

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產品編號 Ys-1374R
英文名稱 BMP4
中文名稱 骨形態(tài)發(fā)生蛋白4抗體
別    名 BMP 4; BMP2B; BMP2B1; Bone morphogenetic protein 4; DVR4; ZYME; BMP4_HUMAN; BMP-4; Bone morphogenetic protein 2B; BMP-2B.  

 


抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應 Mouse, Sheep,  (predicted: Human, Rat, )
產品應用 WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 (石蠟切片需做抗原修復)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 13/45kDa
細胞定位 細胞外基質 分泌型蛋白 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human BMP4: 251-350/408 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed


骨形態(tài)發(fā)生蛋白-4(BMP4)是轉化生長因子β超家族成員,也參與器官發(fā)生,細胞增殖、分化及凋亡等。
產品圖片
Sample:
Sheep skin (Sheep) Lysate at 40 ug
Primary: Anti-BMP4 (bs-1374R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 13/45 kD
Observed band size: 50 kD
Paraformaldehyde-fixed, paraffin embedded (mouse pancreas tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (BMP4) Polyclonal Antibody, Unconjugated (bs-1374R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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