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內(nèi)皮脂肪酶抗體

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產(chǎn)品編號 Ys-2397R
英文名稱 Endothelial lipase
中文名稱 內(nèi)皮脂肪酶抗體
別    名 LIPG; EC 3.1.1.3; EDL; EL; Endothelial cell-derived lipase; Endothelial lipase; Endothelial lipase precursor; Lipase endothelial; LIPE_HUMAN; LIPG; Lipoprotein lipase H; PL; PRO719.  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號 55kDa
交叉反應 Mouse, Rat,  (predicted: Human, Chicken, Dog, Pig, Cow, Horse, )
產(chǎn)品應用 WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (石蠟切片需做抗原修復)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 55kDa
細胞定位 分泌型蛋白 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human Endothelial lipase: 301-400/500 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 The protein encoded by this gene has substantial phospholipase activity and may be involved in lipoprotein metabolism and vascular biology. This protein is designated a member of the TG lipase family by its sequence and characteristic lid region which provides substrate specificity for enzymes of the TG lipase family. [provided by RefSeq, Jul 2008].

Function:
Has phospholipase and triglyceride lipase activities. Hydrolyzes high density lipoproteins (HDL) more efficiently than other lipoproteins. Binds heparin.

Subunit:
Head to tail homodimer.

Subcellular Location:
Secreted.

Tissue Specificity:
High level of expression in the liver, placenta, lung, thyroid, kidney, testis and in the corpus luteum of the ovary. Expressed also in coronary artery endothelial cells, umbilical vein endothelial cells and in hepatocytes and osteosarcoma cell lines. Not detected in heart, brain and muscle.

Similarity:
Belongs to the AB hydrolase superfamily. Lipase family.
Contains 1 PLAT domain.

SWISS:
Q9Y5X9

Gene ID:
9388

Database links:

 



EL被確定為甘油三酯脂肪酶家族的新成員,由于EL是由血管內(nèi)皮細胞合成和分泌,因此被命名為內(nèi)皮脂肪酶,相應的基因命名為內(nèi)皮脂肪酶基因(endothelial lipase gene,LIPG)。EL也是一種分泌型蛋白,由483個氨基酸組成,分子量約55kD,在人體多種組織中都有表達:冠狀動脈、胎盤、甲狀腺、肝臟、肺臟、腎臟、睪丸和卵巢等組織中都可發(fā)現(xiàn)。
產(chǎn)品圖片
Sample: Placenta (Mouse) Lysate at 40 ug
Primary: Anti- Endothelial lipase (bs-2397R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55 kD
Observed band size: 55 kD
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