| 產(chǎn)品編號 | Ys-1002R |
| 英文名稱 | MGMT |
| 中文名稱 | O6甲基鳥嘌呤DNA甲基轉(zhuǎn)移酶抗體 |
| 別 名 | 6 O methylguanine DNA methyltransferase; Agat; AGT; AI267024; EC 2.1.1.63; Methylated DNA protein cysteine methyltransferase; Methylguanine DNA methyltransferase; MGC107020; MGMT: O-6 methylguanine DNA methyltransferase; O 6 methylguanine DNA alkyltransferase; O 6 methylguanine DNA methyltransferase; O-6 methylguanine DNA alkyltransferase; O-6-methylguanine-DNA methyltransferase; O6 methylguanine DNA methyltransferase; MGMT_HUMAN. |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Rat, (predicted: Human, Mouse, ) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復(fù)） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 22kDa |
| 細(xì)胞定位 | 細(xì)胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human MGMT: 101-207/207 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 | MGMT (O6 methylguanine DNA methyltransferase) is an enzyme that transfers methyl groups from O(6) methylguanine, and other methylated moities of DNA, to a cysteine residue in itself, thus repairing DNA damage caused by alkylation in a single step reaction. This is a suicide reaction as the enzyme is irreversibly inactivated. MGMT prevents mutagenesis and malignant transformation, and also provides resistance to chemotherapy with alkylating agents among cancer patients. Function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated. Subcellular Location: Nucleus. Similarity: Belongs to the MGMT family. SWISS: P16455 Gene ID: 4255 Database links:
O-6-methylguanine-DNA methyltransferase(MGMT)O-6-甲基鳥嘌呤-DNA-甲基轉(zhuǎn)移酶:是廣泛存在于細(xì)菌到哺乳動物機(jī)體中的一種獨特DNA修復(fù)酶，MGMT是一種DNA修復(fù)酶，是突變和癌變的抑制因子。它對烷化劑導(dǎo)致的DNA損傷有修復(fù)作用。 MGMT是突變和癌基因的抑制因子。研究表明MGMT水平與惡性腫瘤的發(fā)生、進(jìn)展、浸潤、預(yù)后及治療有密切關(guān)系。細(xì)胞中MGMT水平與其所能耐受的DNA損傷密切相關(guān)。一般來講，低水平表達(dá)MGMT的腫瘤可以應(yīng)用烷化劑藥物治療；反之則為耐藥。在正常組織中，肝臟高表達(dá)；腦組織和骨髓低水平表達(dá)。 |
| 產(chǎn)品圖片 | Tissue/cell: rat liver tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-MGMT Polyclonal Antibody, Unconjugated(bs-1002R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-MGMT Polyclonal Antibody, Unconjugated(bs-1002R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |
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