| 產(chǎn)品編號(hào) | Ys-0200R |
| 英文名稱 | PACAP-38 |
| 中文名稱 | 腺苷酸環(huán)化酶激活肽-38抗體 |
| 別 名 | HSPC190; Proapoptotic Caspase Adaptor Protein; PACAP; pERp1; Plasma cell-induced ER protein 1; Plasma cell-induced resident endoplasmic reticulum protein; Plasma cell-induced resident ER protein; Proapoptotic caspase adapter protein; pituitary adenylate cyclase activating polypeptide; PRP-48; PACAP-related peptide; Pituitary adenylate cyclase-activating polypeptide 38; Pituitary adenylate cyclase-activating polypeptide 38; PACAP38; PACA_HUMAN. |
| 抗體來(lái)源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human, Mouse, Rat, (predicted: Pig, Cow, Sheep, ) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復(fù)） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 5kDa |
| 細(xì)胞定位 | 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human PACAP-38: 31-38/38 (162-139/176) |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 | Pituitary adenylate cyslase Activating polypeptide-27/38, PACAP-27/38 is a neuropeptide that has substantial sequence homology to vasoactive intestinal peptide (VIP).Both VIPand PACAP-38are equipotent at the VCAP-2 receptor, while PAC AP -38 is equipotent to PACAP-27 and more potent than VIP at the PAC-1receptor. The function of PACAP is Stimulates adenylate cyclase in pituitary cells. It belongs to the glucagon family. Function: Binding to its receptor activates G proteins and stimulates adenylate cyclase in pituitary cells. Subunit: Interacts with ADCYAP1R1 (via N-terminal extracellular domain). Subcellular Location: Secreted. Similarity: Belongs to the glucagon family. SWISS: P18509 Gene ID: 116 腺苷酸環(huán)化酶激活肽是一種可激活垂體腺苷酸環(huán)化酶的多肽，有PACAP-27和 PACAP-38兩種形式，他們共同具有N端27個(gè)氨基酸，屬于PACAP/glucagon超級(jí)家族。在中樞神經(jīng)系統(tǒng)分布廣泛，其中以下丘腦含量最高。 |
| 產(chǎn)品圖片 |  Sample: Lane 1: Mouse Pituitary Lysates Primary: Anti-PACAP-38 (bs-0200R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 5kDa Observed band size: 19kDa  Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-PACPA Polyclonal Antibody, Unconjugated(bs-0200R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining  Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-PACAP-38 Polyclonal Antibody, Unconjugated(bs-0200R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining  Tissue/cell: mouse small intestine tissue;4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-PACAP-38 Polyclonal Antibody, Unconjugated(bs-0200R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei |
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