| 產(chǎn)品編號 | Ys-1031R |
| 英文名稱 | IFITM1 |
| 中文名稱 | 干擾素誘導(dǎo)跨膜蛋白1抗體 |
| 別 名 | CD225; CD225 antigen; CD 225; CD-225; IFI17; Interferon induced protein 17; Interferon induced transmembrane protein 1; Interferon inducible protein 9-27; Interferon-induced protein 17; Interferon-induced transmembrane protein 1; Interferon-inducible protein 9-27; Leu 13 antigen; Leu-13 antigen; LEU13; IFM1_HUMAN; Dispanin subfamily A member 2a; DSPA2a. |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human, (predicted: Mouse, Rat, ) |
| 產(chǎn)品應(yīng)用 | ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復(fù)） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 11.7kDa |
| 細(xì)胞定位 | 細(xì)胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human IFITM1: 58-86/125 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 | IFITM1 expression is induced by interferons alpha and gamma and it is thought to play a role in control of cell growth. It is upregulated in several tumor types and may be useful as a tumor biomarker. Function: IFN-induced antiviral protein that mediate cellular innate immunity to at least three major human pathogens, namely influenza A H1N1 virus, West Nile virus, and dengue virus by inhibiting the early step(s) of replication. Plays a key role in the antiproliferative action of IFN-gamma either by inhibiting the ERK activation or by arresting cell growth in G1 phase in a p53-dependent manner. Implicated in the control of cell growth. Component of a multimeric complex involved in the transduction of antiproliferative and homotypic adhesion signals. Subunit: Interacts with CAV1; this interaction enhances the ability of CAV1 in inhibiting ERK activation. Subcellular Location: Cell membrane; Multi-pass membrane protein. Post-translational modifications: Palmitoylation on membrane-proximal cysteines controls clustering in membrane compartments and antiviral activity against influenza virus. Similarity: Belongs to the CD225 family. SWISS: P13164 Gene ID: 8519 IFITM1蛋白是蛋白復(fù)合物的組分之一,主要功能是參與同型粘附和抗增殖信號的轉(zhuǎn)導(dǎo),與細(xì)胞增殖、腫瘤分化、抗病毒、免疫監(jiān)視等有關(guān)。IFITM-1主要用于消化系統(tǒng)腫瘤方面的研究，有學(xué)者認(rèn)為：干擾素誘導(dǎo)跨膜蛋白1與干擾素結(jié)合，可以抑制腫瘤細(xì)胞的分化和增值。 |
| 產(chǎn)品圖片 | Tissue/cell: human glioma tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-IFITM1/CD225 Polyclonal Antibody, Unconjugated(bs-1031R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining  Blank control: Raji (blue). Primary Antibody: Rabbit Anti-IFITM1 antibody(bs-1031R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange),used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol The cells were fixed with 2% paraformaldehyde (10 min) . Primary antibody (bs-1031R, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed. |
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