| 產品編號 | Ys-2553R |
| 英文名稱 | CXCL13 |
| 中文名稱 | B-淋巴細胞趨化因子抗體 |
| 別 名 | ANGIE; ANGIE2; B cell attracting chemokine 1; B cell-attracting chemokine 1; B lymphocyte chemoattractant; B-cell chemoattractant; B-cell-attracting chemokine 1; BCA-1; BLC; BLR1L; C-X-C motif chemokine 13; Chemokine (C-X-C motif) ligand 13; Chemokine (C-X-C motif) ligand 13 (B-cell chemoattractant); Chemokine, CXC motif, ligand 13; CXC chemokine BLC; CXCL13; CXL13_HUMAN; SCYB13; Small inducible cytokine B subfamily (Cys-X-Cys motif), member 13 (B-cell chemoattractant); Small inducible cytokine B13; Small inducible cytokine subfamily B, member 13; Small-inducible cytokine B13. |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human, (predicted: Dog, ) |
| 產品應用 | WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1ug/Test IF=1:100-500 （石蠟切片需做抗原修復） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 10kDa |
| 細胞定位 | 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CXCL13: 23-109/109 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 | B lymphocyte chemoattractant, independently cloned and named Angie, is a CXC chemokine strongly expressed in the follicles of the spleen, lymph nodes, and Peyer's patches. It preferentially promotes the migration of B lymphocytes (compared to T cells and macrophages), apparently by stimulating calcium influx into, and chemotaxis of, cells expressing Burkitt's lymphoma receptor 1 (BLR1). It may therefore function in the homing of B lymphocytes to follicles. Function: Chemotactic for B-lymphocytes but not for T-lymphocytes, monocytes and neutrophils. Does not induce calcium release in B-lymphocytes. Binds to BLR1/CXCR5. Subcellular Location: Secreted. Tissue Specificity: Highest levels in liver, followed by spleen, lymph node, appendix and stomach. Low levels in salivary gland, mammary gland and fetal spleen. Similarity: Belongs to the intercrine alpha (chemokine CxC) family. SWISS: O43927 Gene ID: 10563 |
| 產品圖片 |  Sample: Lane 1: Human K562 cell Lysates Primary: Anti-CXCL13 (bs-2553R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 10kDa Observed band size: 10kDa  Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CXCL13) Polyclonal Antibody, Unconjugated (bs-2553R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Image was kindly submitted by Erica Pimenta from UMDNJ. Human breast tumor tissue stained with Rabbit Anti-CXCL13 Polyclonal Antibody (bs-2553R)  Blank control（black line）:HepG2. Primary Antibody (green line): Rabbit Anti-CXCL13 antibody (bs-2553R) Dilution:1ug/Test; Secondary Antibody（white blue line）: Goat anti-rabbit IgG-AF488 Dilution: 0.5ug/Test. Isotype control（orange line）: Normal Rabbit IgG Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. |
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