| 產(chǎn)品編號 | Ys-2503R |
| 英文名稱 | EEF1A1 |
| 中文名稱 | 翻譯延伸因子EF-1a抗體 |
| 別 名 | Elongation factor 1-alpha 1; EF-1-alpha-1; Elongation factor Tu (EF-Tu); Eukaryotic elongation factor 1 A-1 (eEF1A-1); Leukocyte receptor cluster member 7; EF1A1_HUMAN; EEF1A; EF1A; LENG7; |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human, Mouse, Rat, (predicted: Chicken, Dog, Pig, Cow, Horse, Rabbit, ) |
| 產(chǎn)品應用 | WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 50kDa |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human EEF1A1: 131-230/462 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 | This gene encodes an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas, and the other isoform (alpha 2) is expressed in brain, heart and skeletal muscle. This isoform is identified as an autoantigen in 66% of patients with Felty syndrome. This gene has been found to have multiple copies on many chromosomes, some of which, if not all, represent different pseudogenes. [provided by RefSeq, Jul 2008] Function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. Subcellular Location: Cytoplasm. Tissue Specificity: Brain, placenta, lung, liver, kidney, pancreas but barely detectable in heart and skeletal muscle Post-translational modifications: ISGylated. Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily. SWISS: P68104 Gene ID: 1915 肽鏈延長因子1A-1(peptide elongation factor 1A-1,eEF1A-1)是真核細胞蛋白翻譯過程中肽鏈延長復合物的重要構(gòu)成成分,具有調(diào)節(jié)蛋白合成及促發(fā)凋亡等多種作用. |
| 產(chǎn)品圖片 |  Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-eEF1A1 Polyclonal Antibody, Unconjugated(bs-2503R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining  Tissue/cell: Mouse embryos; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-EF1a/eEF1A1 Polyclonal Antibody, Unconjugated(bs-2503R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |
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