| 產(chǎn)品編號 | Ys-1143R |
| 英文名稱 | STAT6 |
| 中文名稱 | 信號轉(zhuǎn)導(dǎo)和轉(zhuǎn)錄激活因子6抗體 |
| 別 名 | signal transducers and activators of transduction 6; D12S1644; IL 4 STAT; IL4 STAT; Interleukin 4 Induced; Interleukin 4 Induced Transcription Factor IL4 STAT; Signal Transducer And Activator Of Transcription 6 Interleukin 4 Induced; Signal Transducer And Activator Of Transcription 6 Nirs Variant 1; STAT 6; STAT interleukin4 induced; STAT6B; STAT6C; Transcription factor IL 4 STAT. |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human, Mouse, Rat, |
| 產(chǎn)品應(yīng)用 | ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復(fù)） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 94kDa |
| 細(xì)胞定位 | 細(xì)胞核 細(xì)胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human STAT6: 601-700/841 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 | The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein plays a central role in exerting IL4 mediated biological responses. It is found to induce the expression of BCL2L1/BCL-X (L), which is responsible for the anti-apoptotic activity of IL4. Knockout studies in mice suggested the roles of this gene in differentiation of T helper 2 (Th2) cells, expression of cell surface markers, and class switch of immunoglobulins. Function: Carries out a dual function: signal transduction and activation of transcription. Involved in interleukin-4 signalling. Subunit: Forms a homodimer or a heterodimer with a related family member. Interacts with NCOA1 via its C-terminal LXXLL motif. Subcellular Location: Cytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation. Post-translational modifications: Tyrosine phosphorylated following stimulation by IL-4 and IL-3. Similarity: Belongs to the transcription factor STAT family. Contains 1 SH2 domain. SWISS: P42226 Gene ID: 6778 信息轉(zhuǎn)導(dǎo)和轉(zhuǎn)錄激活因子-6(signal transducer and activator of transcription-6，STAT6)是信號轉(zhuǎn)導(dǎo)和轉(zhuǎn)錄激活因子(STAT)家族的一員，是一種94kD的蛋白質(zhì)。它通過JAK-STAT信號轉(zhuǎn)導(dǎo)途徑介導(dǎo)細(xì)胞因子IL-3、IL-4及IL-13誘導(dǎo)的基因表達(dá)，特別是在促進(jìn)IL-4誘導(dǎo)的MHCⅡ類抗原及免疫球蛋白受體等的表達(dá)及Th2細(xì)胞和淋巴細(xì)胞的發(fā)育上發(fā)揮了重要的作用。 |
| 產(chǎn)品圖片 |  Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (STAT6) Polyclonal Antibody, Unconjugated (bs-1143R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.  Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (STAT6) Polyclonal Antibody, Unconjugated (bs-1143R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.  Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (STAT6) Polyclonal Antibody, Unconjugated (bs-1143R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.  Paraformaldehyde-fixed, paraffin embedded (Human stomach); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxidee for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (STAT6) Polyclonal Antibody, Unconjugated (bs-1143R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.  Blank control:Hela. Primary Antibody (green line): Rabbit Anti-STAT6 antibody (bs-1143R) Dilution: 1ug/Test; Secondary Antibody : Goat anti-rabbit IgG-FITC Dilution: 0.5ug/Test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.  Blank control: A549. Primary Antibody (green line): Rabbit AntiSTAT6 antibody (bs-1143R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 3μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. |
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