| 產(chǎn)品編號(hào) | Ys-2052R |
| 英文名稱 | Transferrin(Serum Loading Control) |
| 中文名稱 | 轉(zhuǎn)鐵蛋白/血清鐵傳遞蛋白（內(nèi)參）抗體 |
| 別 名 | Apotransferrin; TRF; eta 1 metal binding globulin; DKFZp781D0156; PRO1400; PRO1557; PRO2086; Serotransferrin precursor; Siderophilin; TF; TRFE_HUMAN; Beta-1 metal-binding globulin; Serotransferrin. |
| 產(chǎn)品類型 | 內(nèi)參抗體 |
| 研究領(lǐng)域 | 心血管 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human, Mouse, Rat, Goat, |
| 產(chǎn)品應(yīng)用 | WB=1:1000-3000 ELISA=1:5000-10000 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 77kDa |
| 細(xì)胞定位 | 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | Full length native Transferrin protein purified from human plasma |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 | This gene encodes a glycoprotein with an approximate molecular weight of 76.5 kDa. It is thought to have been created as a result of an ancient gene duplication event that led to generation of homologous C and N-terminal domains each of which binds one ion of ferric iron. The function of this protein is to transport iron from the intestine, reticuloendothelial system, and liver parenchymal cells to all proliferating cells in the body. This protein may also have a physiologic role as granulocyte/pollen-binding protein (GPBP) involved in the removal of certain organic matter and allergens from serum. [provided by RefSeq, Sep 2009]. Function: Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation. Subunit: Monomer. Subcellular Location: Secreted. Tissue Specificity: Expressed by the liver and secreted in plasma. DISEASE: Defects in TF are the cause of atransferrinemia (ATRAF) [MIM:209300]. Atransferrinemia is rare autosomal recessive disorder characterized by iron overload and hypochromic anemia. Similarity: Belongs to the transferrin family. Contains 2 transferrin-like domains. SWISS: P02787 Gene ID: 7018 |
| 產(chǎn)品圖片 |  Sample: Lane 1: Serum (Human) at 20 ug Lane 2: Serum (Mouse) at 20 ug Lane 3: Serum (Rat) at 20 ug Lane 4: Serum (Goat) at 20 ug Primary: Anti- Transferrin (bs-2052R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 77 kD Observed band size: 77 kD  Sample: Lane 1: Serum (Human) at 20 ug Primary: Anti-Transferrin (bs-2052R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 77 kD Observed band size: 77 kD  Blank control（black line）:HepG2. Primary Antibody (green line): Rabbit Anti-Transferrin antibody (bs-2052R) Dilution:1ug/Test; Secondary Antibody : Goat anti-rabbit IgG-AF488 Dilution: 0.5ug/Test. Negative control（white blue line）: PBS Isotype control（orange line）: Normal Rabbit IgG Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.  Blank control（black line）:HepG2. Primary Antibody (green line): Rabbit Anti-Phospho-MYPT1 (Thr696) antibody (bs-2052R) Dilution:1ug/Test; Secondary Antibody : Goat anti-rabbit IgG-AF488 Dilution: 0.5ug/Test. Negative control（white blue line）: PBS Isotype control（orange line）: Normal Rabbit IgG Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. |
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