| 產(chǎn)品編號 | Ys-0661R |
| 英文名稱 | LRP/MVP |
| 中文名稱 | 肺耐藥相關(guān)蛋白抗體 |
| 別 名 | 2310009M24Rik; LRP; Lung Re |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human, (predicted: Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Guinea Pig, ) |
| 產(chǎn)品應(yīng)用 | ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=2μg/Test ICC=1:100 IF=1:100-500 （石蠟切片需做抗原修復(fù)） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 99kDa |
| 細(xì)胞定位 | 細(xì)胞核 細(xì)胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human LRP: 88-180/893 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 | This gene encodes the major component of the vault complex. Vaults are multi-subunit ribonucleoprotein structures that may be involved in nucleo-cytoplasmic transport. The encoded protein may play a role in multiple cellular processes by regulating the MAP kinase, JAK/STAT and phosphoinositide 3-kinase/Akt signaling pathways. The encoded protein also plays a role in multidrug resistance, and expression of this gene may be a prognostic marker for several types of cancer. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, May 2012] Function: Required for normal vault structure. Vaults are multi-subunit structures that may act as scaffolds for proteins involved in signal transduction. Vaults may also play a role in nucleo-cytoplasmic transport. Down-regulates INFG-mediated STAT1 signaling and subsequent activation of JAK. Down-regulates SRC activity and signaling through MAP kinases. Subunit: The vault ribonucleoprotein particle is a huge (400 A x 670 A) cage structure of 12.9 MDa. It consists of a dimer of half-vaults, with each half-vault comprising 39 identical major vault protein (MVP) chains, PARP4 and one or more vault RNAs (vRNAs). Interacts with TEP1. Interacts with PTEN and activated MAPK1. The phosphorylated protein interacts with the SH2 domains of PTPN11 and SRC. Interacts with APEX1. May interact with ZNF540. Subcellular Location: Cytoplasm. Nucleus, nuclear pore complex. Tissue Specificity: Present in most normal tissues. Higher expression observed in epithelial cells with secretory and excretory functions, as well as in cells chronically exposed to xenobiotics, such as bronchial cells and cells lining the intestine. Overexpressed in many multidrug-resistant cancer cells. Post-translational modifications: Phosphorylated on Tyr residues after EGF stimulation. Dephosphorylated by PTPN11. Similarity: Contains 9 MVP (vault) repeats. SWISS: Q14764 Gene ID: 9961 LRP或稱MVP(Major vault protein)為肺耐藥相關(guān)蛋白。其耐藥機制與該蛋白質(zhì)參與細(xì)胞核-漿的運輸有關(guān)。 他在正常細(xì)胞有廣泛分布，對多種藥物耐藥的癌細(xì)胞呈過度表達，亦屬多藥耐藥(MDR)中的一種表型，可表達于許多腫瘤組織中，陽性者預(yù)示對化療不敏感，主要用于各種惡性腫瘤的研究。 |
| 產(chǎn)品圖片 |  Paraformaldehyde-fixed, paraffin embedded (Human colon cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (LRP/MVP) Polyclonal Antibody, Unconjugated (bs-0661R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.  Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (LRP/MVP) polyclonal Antibody, Unconjugated (bs-0661R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.  The figure annotation: The blue histogram is unstained cells. The Orange histogram is cells stained with Rabbit IgG/FITC (bs-0295P-FITC) The green histogram is cells stained with Rabbit Anti-LRP/MVP/FITC Conjugated antibody (bs-0661R-FITC). Controls Positive control: HepG 2 cells Isotype control: Cell lines treated with Rabbit IgG/FITC (bs-0295P-FITC) instead of the primary antibody to confirm that primary antibody binding is specific. 2μg in 1 00 μL 1 X PBS containing 0.5% BSA. |
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