| 產(chǎn)品編號(hào) | Ys-21617R |
| 英文名稱 | SFTPD |
| 中文名稱 | 肺表面活性蛋白D抗體 |
| 別 名 | SFTPD surfactant protein D; pulmonary surfactant-associated glycoprotein D; COLEC 7; COLEC7; Collectin7; PSP D; PSPD; Pulmonary surfactant apoprotein; Pulmonary surfactant associated protein D; SFTP 4; SFTP4; SFTPD; SP D; Surfactant associated protein pulmonary 4; Surfactant pulmonary associated protein D; SFTPD_HUMAN; Pulmonary surfactant-associated protein D; PSP-D; SP-D; Collectin-7; Lung surfactant protein D. |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human, (predicted: Mouse, Rat, ) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=2ug/Test ICC=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復(fù)） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 41kDa |
| 細(xì)胞定位 | 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from mouse SFTPD: 301-400/374 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 | Surfactant protein D contributes to the lung's defense against inhaled microorganisms. It's a calcium ion dependent carbohydrate binding protein and is structurally similar to other C type mammalian lectins, such as conglutinin and SPA. It has a molecular size of approximately 37-43kDa in its reduced state, 620kDa in non dissociating conditions. Surfactant protein D enhances the production of oxygen radicals by rat alveolar macrophages and regulates some actions of SPA, which is the most abundant surfactant protein. Surfactant protein D is synthesized and secreted by alveolar epithelial type II cells. Function: Contributes to the lung's defense against inhaled microorganisms. May participate in the extracellular reorganization or turnover of pulmonary surfactant. Binds strongly maltose residues and to a lesser extent other alpha-glucosyl moieties. Subunit: Oligomeric complex of 4 set of homotrimers. Subcellular Location: Secreted, extracellular space, extracellular matrix. Secreted, extracellular space, surface film. Post-translational modifications: The N-terminus is blocked. Hydroxylation on proline residues within the sequence motif, GXPG, is most likely to be 4-hydroxy as this fits the requirement for 4-hydroxylation in vertebrates. Similarity: Belongs to the SFTPD family. Contains 1 C-type lectin domain. Contains 1 collagen-like domain. SWISS: P50404 Gene ID: 20390 |
| 產(chǎn)品圖片 |  Sample: Lane 1: MCF-7 (Human) Cell Lysate at 30 ug Lane 2: SH-SY5Y (Human) Cell Lysate at 30 ug Lane 3: 293T (Human) Cell Lysate at 30 ug Lane 4: A549 (Human) Cell Lysate at 30 ug Primary: Anti-SFTPD (bs-21617R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 43/50 kD Observed band size: 50 kD  Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SFTPD) Polyclonal Antibody, Unconjugated (bs-21617R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.  Blank control:THP-1. Primary Antibody (green line): Rabbit Anti-SFTPD antibody (bs-21617R) Dilution: 2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-FITC Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. |
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