| 產(chǎn)品編號(hào) | Ys-1163R |
| 英文名稱 | Defensin beta 1 |
| 中文名稱 | 防御素β1/Defensin β1抗體 |
| 別 名 | BD 1; BD-1; BD1; beta 1; Beta defensin 1; Beta-defensin 1; DEFB 1; DEFB1; DEFB1_HUMAN; DEFB101; Defensin; Defensin beta 1 preproprotein; HBD 1; hBD-1; HBD1; MGC51822. |
| 研究領(lǐng)域 | 細(xì)胞生物 |
| 抗體來(lái)源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human, Mouse, Rat, |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復(fù)） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 4.1/8kDa |
| 細(xì)胞定位 | 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from rat Defensin Beta 1: 33-68/68 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 | Defensins form a family of microbicidal and cytotoxic peptides made by neutrophils. Members of the defensin family are highly similar in protein sequence. This gene encodes defensin, beta 1, an antimicrobial peptide implicated in the resistance of epithelial surfaces to microbial colonization. This gene maps in close proximity to defensin family member, defensin, alpha 1 and has been implicated in the pathogenesis of cystic fibrosis. The mature form of Beta defensin 1 is 36 amino acids. Function: Has bactericidal activity. Subcellular Location: Secreted. Tissue Specificity: Plasma. Similarity: Belongs to the beta-defensin family. SWISS: O89117 Gene ID: 64389 Database links:
防御素在動(dòng)物體中廣泛分布的，目前已知的α－防御素有6種，研究人員從動(dòng)物體的腎臟、氣管、鼻粘膜與唾液中也發(fā)現(xiàn)了兩種β防御素，在許多器官的上皮細(xì)胞與非上皮組織的細(xì)胞中發(fā)現(xiàn)了β－防御素。防御素的表達(dá)與病原體的入侵是緊密相關(guān)。 |
| 產(chǎn)品圖片 |  Sample: Lane 1: Mouse trachea tissue lysates Lane 2: Mouse Kidney tissue lysates Lane 3: Rat trachea tissue lysates Lane 4: Rat Kidney tissue lysates Primary: Anti-Defensin beta 1 (bs-1163R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 4.1/8 kDa Observed band size: 12 kDa  Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-beta Defensin 1 Polyclonal Antibody, Unconjugated(bs-1163R) 1:400, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Defensin β1 Polyclonal Antibody, Unconjugated(bs-1163R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Defensin β1 Polyclonal Antibody, Unconjugated(bs-1163R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |
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