| 產(chǎn)品編號 | Ys-1806R |
| 英文名稱 | GPR14 |
| 中文名稱 | G蛋白偶聯(lián)受體14抗體 |
| 別 名 | G protein coupled receptor 14; Urotensin II receptor (UR II R); UTR; UTR2; UTS2R; G Protein Coupled Receptor 14; GPCR GPR14; Urotensin II receptor; UR-II-R; UR-2-R; G-protein coupled receptor 14; G-protein coupled sensory epithelial neuropeptide-like receptor; SENR; GPR14; UR2R_HUMAN; GPCR GPR14. |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human, Mouse, (predicted: Rat, ) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000 ELISA=1:5000-10000 IHC-F=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復(fù)） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 42kDa |
| 細(xì)胞定位 | 細(xì)胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human UTS2R: 101-200/389 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 | GPCR GPR14 has been reported to be expressed at high levels in the heart and pancreas and also in artery and smooth muscle. Low transcript levels have been identified in brain, particularly in thalamus, superior occipital gyrus, and substantia nigra. GPR14 is a high affinity receptor for urotensin-2 and urotensin-2B. The activity of this receptor is mediated by a G-protein that activates a phosphatidylinositol-calcium second messenger system. Referenced from Swiss-Prot Q9UKP6. Function: High affinity receptor for urotensin-2 and urotensin-2B. The activity of this receptor is mediated by a G-protein that activate a phosphatidylinositol-calcium second messenger system. Subcellular Location: Cell membrane; Multi-pass membrane protein. Tissue Specificity: Most abundant expression in the heart and pancreas. Similarity: Belongs to the G-protein coupled receptor 1 family. SWISS: Q9UKP6 Gene ID: 2837 |
| 產(chǎn)品圖片 |  Sample: Heart (Mouse) Lysate at 40 ug Primary: Anti-GPR14 (bs-1806R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 42 kD Observed band size: 42 kD  Blank control:U937. Primary Antibody (green line): Rabbit Anti-GPR14 antibody (bs-1806R) Dilution: 2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol The cells wereincubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. |
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