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Purified anti-human CD117 (c-kit) Antibody

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Product Details

Verified Reactivity
Human
Reported Reactivity
Cynomolgus, Cow
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
MOLM-1 megakaryocytic cell line
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
CyTOF? - Verified
ICC, IP - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The 104D2 antibody does not block binding of c-Kit ligand. Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunofluorescence microscopy1, and spatial biology (IBEX)4,5.

Application References

(PubMed link indicates BioLegend citation)
  1. Broudy VC, et al. 1999. Blood 94:1979. (IF, IP)
  2. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  3. Nagano M, et al. 2007. Blood 110:151. (FC) PubMed
  4. Radtke　AJ,　et al.　2020.　Proc Natl Acad Sci U S A.　117:33455-65. (SB)　PubMed
  5. Radtke　AJ,　et al.　2022.　Nat Protoc.　17:378-401. (SB)　PubMed
Product Citations
  1. Lowinus T, et al. 2019. Cell Commun Signal. 17:05. PubMed
  2. Ortiz AM, et al. 2018. Nat Med. 24:1313. PubMed
  3. Vigneault P, et al. 2018. J Physiol. 596:2359. PubMed
  4. Vivanco Gonzalez N, et al. 2022. STAR Protoc. 3:101280. PubMed
  5. Ga?án-Gómez I, et al. 2022. Nat Med. . PubMed
  6. Oeztuerk-Winder F, et al. 2012. EMBO J. 31:3431. PubMed
  7. Baskar R, et al. 2022. Cell Rep Methods. 2:. PubMed
  8. Nagano M, et al. 2007. Blood . 110:151. PubMed
  9. Guo A, et al. 2011. J Pharmacol Exp Ther. 338:421. PubMed
  10. Ardain A, et al. 2019. Nature. 570:528. PubMed
  11. Evrard M et al. 2018. Immunity. 48(2):364-379 . PubMed
  12. Crawford LB, et al. 2021. J Virol. 95:. PubMed
  13. Dinh HQ, et al. 2020. Immunity. 53(2):319-334.e6. PubMed
  14. Cytlak U, et al. 2020. Immunity. 53(2):353-370. PubMed
  15. Leylek R, et al. 2020. Cell Rep. 32:108180. PubMed
  16. Jokela TA, et al. 2018. Front Cell Dev Biol. 6:41. PubMed
RRID
AB_314980 (BioLegend Cat. No. 313201) AB_314981 (BioLegend Cat. No. 313202)

Antigen Details

Structure
Growth factor receptor with tyrosine kinase activity, subclass III, approximately 145 kD
Distribution

Pluripotent hematopoietic progenitor cells (approximately 1-4% bone marrow cells), mast cells, acute myeloid leukemic cells (AML)

Function
Growth factor receptor for stem cell factor. Induces proliferation and survival of primitive hematopoietic progenitors. Potent inducer of proliferation in erythroid-committed progenitor cells. Defects in CD117 have been linked to severe anemia and a decreased number of hematopoietic progenitor cells.
Ligand/Receptor
c-Kit ligand
Modification
Multiple phosphorylation sites
Cell Type
Embryonic Stem Cells, Hematopoietic stem and progenitors, Leukemia, Mast cells, Mesenchymal Stem Cells
Biology Area
Immunology, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. Giebel LB, et al. 1992. Oncogene 7:2207.
2. Furitsu T, et al. 1993. J. Clin. Invest. 92:1736.

Gene ID
3815 View all products for this Gene ID
UniProt
View information about CD117 on UniProt.org
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