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Alexa Fluor® 647 anti-mouse/human A2B5 Antibod

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Product Details

Verified Reactivity
Mouse, Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Embryonic chicken retinal cells.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 647 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats of this clone) include: immunofluorescence staining.1,2

Application References

(PubMed link indicates BioLegend citation)
  1. Eisenbarth GS, et al. 1979. Proc. Natl. Acad. Sci. 76:4913. (IF)
  2. Eisenbarth GS, et al. 1982. Proc. Natl. Acad. Sci. 79:5066. (IF)
  3. Russell TR, et al. 1984. Cytometry 5:539. (FC)
RRID
AB_2566394 (BioLegend Cat. No. 150703) AB_2566395 (BioLegend Cat. No. 150704)

Antigen Details

Distribution
Developing thymic epithelial cells, oligodendrocyte progenitors, neuroendocrine, embryonic neonatal tissue, astrocytoma, glioma, and squamous cell carcinoma.
Cell Type
Epithelial cells, Oligodendrocytes
Biology Area
Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
Antigen References

1. Baracskay KL, et al. 2007. GLIA 55:1001.
2. Raff MC, et al. 1983. J. Neurosci. 6:1289.
3. Fredman P, et al. 1984. Arch. Biochem. Biophys. 2:661.
4. Xia CL, et al. 2003. Int. J. Oncol. 23:353.
5. Tchoghandjian A, et al. Brain Pathol. 20:211.

Gene ID
NA
UniProt
View information about A2B5 on UniProt.org
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