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Purified anti-SSRP1 Antibody

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Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant (partial) (308-524aa)
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide at 0.5 mg/ml.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, store undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC, ChIP - Verified
IP - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 ?g per 5 ml antibody dilution buffer for each mini-gel. For ChIP applications, the suggested dilution is 1:50-1:200 by volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

10D1 displayed a stronger affinity for SSRP1 than BioLegend clone 3E4 when tested for western blot application.

Application References

(PubMed link indicates BioLegend citation)
  1. Tan BC, et al. 2006. EMBO 25:3975. (ChIP)
  2. Carvalho S, et al. 2013. Nucleic Acids Res. 41:2881. PubMed
  3. Tan BC, et al. 2004. J. Biol. Chem. 279:9321. (Immunogen)
  4. Lolis AA, et al. 2013. J Biol Chem. 288:7676. PubMed
  5. Aida M, et al. 2013. PNAS. 110:7784. PubMed
  6. Dermawan JK, et al. 2014. Mol Cancer Ther. 13:2203. PubMed
  7. Shakya A, et al. 2015. Mol Cell Biol. 35:1014. PubMed
Product Citations
  1. Dermawan J, et al. 2016. Cancer Res . 76: 2432-2442. PubMed
  2. Srinivasan R, et al. 2020. Sci Adv. 6:eaaz9115. PubMed
  3. Fleyshman D, et al. 2017. Oncotarget. 8:20525. PubMed
  4. Isa NF, et al. 2021. Vaccines (Basel). 9:. PubMed
  5. Safina A, et al. 2017. Nucleic Acids Res. 10.1093/nar/gkw1366. PubMed
  6. Carvalho S, et al. 2013. Nucleic Acids Res. 41:2881. PubMed
  7. Shakya A, et al. 2015. Mol Cell Biol. 35:1014. PubMed
  8. Li X, et al. 2021. J Biol Chem. 297:101360. PubMed
  9. Wever V, et al. 2012. PLoS One. 7:e39510. PubMed
  10. Sundarraj J, et al. 2021. Wellcome Open Res. 2:83. PubMed
  11. Narain A, et al. 2021. Molecular Cell. 81(15):3110-3127.e14. PubMed
  12. Lolis A, et al. 2013. J Biol Chem. 288:7676. PubMed
  13. Piquet S et al. 2018. Molecular cell. 72(5):888-901 . PubMed
  14. Aida M, et al. 2013. Proc Natl Acad Sci U S A. 110:7784. PubMed
  15. Dermawan J, et al. 2014. Mol Cancer Ther. 13:2203. PubMed
  16. Sandlesh P, et al. 2018. PLoS One. 15:81. PubMed
  17. Ali A, et al. 2019. FASEB J. 33:2621. PubMed
  18. Prendergast L, et al. 2020. Oncogene. 39:5124. PubMed
  19. Paul S, et al. 2018. J Biol Chem. 293:19303. PubMed
  20. Wang AS, et al. 2020. Molecular Cell. 79(2):221-233.e5.. PubMed
RRID
AB_315730 (BioLegend Cat. No. 609701) AB_315731 (BioLegend Cat. No. 609702)

Antigen Details

Structure
SSRP family, HMG box domain; 81 kD
Distribution

Nuclear

Function
Interacts with nucleosomes and histone H2A/H2B dimers to promote nucleosome disassembly and allow transcription elongation, binds to dsDNA. Modified by cisplatin, may protect DNA from repair and block DNA replication
Interaction
Component of FACT ATP-dependent chromatin remodeling complex, SUPT16H/Cdc68 in FACT complex, H2A/H2B dimers
Biology Area
Cell Biology, Chromatin Remodeling/Epigenetics, DNA Repair/Replication, Transcription Factors
Antigen References

1. LeRoy G, et al. 1998. Science 282:1836.
2. Orphanides G, et al. 1998. Cell 92:105.
3. Orphanides G, et al. 1999. Nature 400:284.

Gene ID
6749 View all products for this Gene ID
UniProt
View information about SSRP1 on UniProt.org
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