Product Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Recombinant mouse CD204 extracellular domain
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
- Concentration
- 0.2 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 ?g per million cells in 100 ?L volume. It is recommended that the reagent be titrated for optimal performance for each application.
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.- Excitation Laser
- Blue Laser (488 nm)
- Application Notes
This clone has minimal recognition of CD204 in C57BL/6.
- RRID
- AB_2892313 (BioLegend Cat. No. 154715) AB_2892313 (BioLegend Cat. No. 154716)
Antigen Details
- Structure
- Trimeric type II transmembrane protein, one scavenger receptor cysteine-rich domain (SRCR), 220 kD.
- Distribution
-
Macrophages, dendritic cells.
- Function
- Uptake of negatively charged molecules, role in pathology of atherosclerosis and Alzheimer’s disease.
- Ligand/Receptor
- Acetylated and oxidized lipoproteins
- Cell Type
- Dendritic cells, Macrophages
- Antigen References
-
- Shichita T, et al. 2017. Nat Med. 23:723.
- Muczynski V, et al. 2017. Blood. 129:2443.
- Iftakhar-E-Khuda I, et al. 2016. Proc Natl Acad Sci U S A. 113:10643.
- Nellimarla S, et al. 2015. J Immunol. 195:3858.
- Bonilla DL, et al. 2013. Immunity. 39:537.
- Gene ID
- 20288 View all products for this Gene ID
- UniProt
- View information about CD204 on UniProt.org
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