Product Details
- Verified Reactivity
- E. coli, Klebsiella, Salmonella, Serratia, Bordetella
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant protein
- Preparation
- Dried ascites
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- Store lyophilized antibody at -20°C.
- Application
-
WB - Quality tested
ELISA - Verified
FA - Reported in the literature, not verified in house - Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. To reconstitute, add 100 ?l of ddH2O to the tube containing antibody and mix to dissolve. For Western blotting, the suggested dilution is 1:1000 after reconstitution. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Previously item# W0009 from Neoclone.
Clone 1RS1 does not react with Shigella, Vibrio, Pseudomonas, Rhodobacter, Agrobacterium, Streptococcus, Arthobacter, or Borrelia. It recognizes epitope 33-256.
Additional reported applications (for the relevant formats) include: functional assay.- Additional Product Notes
Non-specific band may be observed at 60 kD.
- Application References
(PubMed link indicates BioLegend citation) -
- Nguyen LH, et al. 1993. Biochemistry 32:11112.
- Product Citations
-
- RRID
- AB_2564522 (BioLegend Cat. No. 663703)
Antigen Details
- Structure
- Sigma S (σS) is a 38 kD transcription factor of E. coli RNA polymerase.
- Biology Area
- Cell Biology, Transcription Factors
- Molecular Family
- Nuclear Markers
- Gene ID
- 947210 View all products for this Gene ID
- UniProt
- View information about Sigma S on UniProt.org
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