Product Details
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Partial human Oct4 recombinant protein (1-141aa).
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 647 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICC - Quality tested
ICFC - Verified - Recommended Usage
Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 2.5 - 5.0 μg/ml is recommended. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per million cells in 100 ?l volume.?It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor? 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses- Excitation Laser
- Red Laser (633 nm)
- Product Citations
-
- RRID
- AB_2566689 (BioLegend Cat. No. 653710)
Antigen Details
- Structure
- Belongs to the POU transcription factor family, predicted molecular weight of 39 kD
- Distribution
-
Nuclear protein, expressed in undifferentiated embryonal carcinoma (EC) cells, embryonic stem (ES) cells and embryonic germ (EG) cells
- Function
- Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Oct4 is a prime candidate for an early developmental control gene. It also is an important marker of the undifferentiated state and central regulator of pluripotency in ES cells.
- Interaction
- SOX2, SOX8, SOX10, androgen receptor, and ETS2
- Biology Area
- Cell Biology, Immunology, Stem Cells, Transcription Factors
- Antigen References
-
1. Niwa H, et al. 2000. Nature Genet. 24:372.
2. Wernig M, et al. 2007. Nature 448:318.
3. Pesce M, et al. 2001. Stem Cells 19:271. - Gene ID
- 5460 View all products for this Gene ID
- UniProt
- View information about Oct4 on UniProt.org
