Product Details
- Verified Reactivity
- Human, Hamster
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Full length human IκB-α recombinant protein expressed in E. coli.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
ICC - Verified - Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 0.5 ?g/ml (1:1000 - 1:5000 dilution).?For immunocytochemistry, the suggested use is 0.5 - 1.0 ?g/ml (1:500-1:1000 dilution). It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
This antibody weakly reacts with mouse and rat. Based on in-house testing, clone 3D6C02 does not block clone 25/IkBa/MAD-3, and only partially blocks L35A5 also raised against I?B-a.
- Product Citations
-
- RRID
- AB_2564258 (BioLegend Cat. No. 662402)
Antigen Details
- Structure
- Inhibitory protein of the NF-κB complex that contains multiple ankyrin repeats.
- Distribution
-
Cytoplasm
- Function
- Regulates transcriptional activity of NF-κB by binding the complex and trapping it in the cytoplasm. Serine phosphorylation of IκB targets it for destruction via the ubiquitination pathway, which allows the NF-κB complex to translocate to the nucleus.
- Interaction
- NFkB1, SUMO4, p53, ribosomal S6 kinase 1
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Signal Transduction
- Molecular Family
- Tumor Suppressors
- Antigen References
-
1. Baeuerle PA. 1998. Cell 95:729.
2. Hoffmann A, et al. 2002. Science 298:1241.
3. Ito CY, et al. 1995. Genomics 29:490. - Gene ID
- 4792 View all products for this Gene ID
- UniProt
- View information about IkappaB-alpha on UniProt.org
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