Product Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant mouse FBXL5
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.1 - 1.0 ?g/mL. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
This clone was tested for ICC on PFA-fixed, Triton X-100-permeabilized NIH/3T3 cells. The clone produced nuclear staining that did not match the iron-responsiveness observed during western blotting. Because of this, we do not recommend using this clone for IF.
During product development testing, a protein with a slightly lower molecular weight than the target was observed during PD testing. This protein displayed a similar iron-responsiveness to the target band. It is unclear whether this band represents a minor FBXL5 degradation product or a truncated isoform.
This clone does not recognize human FBXL5. For detection of human FBXL5 by western blot, we recommend BioLegend clone 10F4H9D12 (Cat. No. 672604).
This clone has not been tested for IP.- RRID
- AB_2832845 (BioLegend Cat. No. 672703) AB_2832846 (BioLegend Cat. No. 672704)
Antigen Details
- Structure
- FBXL5 is a 690 amino acid protein with a predicted molecular weight of 78.56 kD.
- Distribution
-
Cytosol and nucleus/ubiquitously expressed
- Function
- Regulates iron homeostasis
- Antigen References
-
- Johnson NB, et al. 2017. J. Biol. Chem. 292:15976.
- Vinas-Castells R, et al. 2014. Nucleic Acids Res. 42:1079.
- Moroishi T, et al. 2014. J. Biol. Chem. 289:16430.
- Ruiz JC, et al. 2013. J. Biol. Chem. 288:552.
- Chollangi S, et al. 2012. J. Biol. Chem. 287:23710.
- Moroishi T, et al. 2011. Cell Metab. 14:339.
- Salahudeen AA, et al. 2009 Science. 326:722.
- Vashisht AA, et al. 2009 Science. 326:718.
- Gene ID
- 242960 View all products for this Gene ID
- UniProt
- View information about FBXL5 on UniProt.org
