Product Details
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Partial human PTEN recombinant protein (303-403aa)
- Formulation
- This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.05% sodium azide.
- Preparation
- Affinity purified.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- Upon receipt, store undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
ICC - Verified - Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.5 ?g per ml. For immunocytochemistry, a concentration range of 2.5 - 5.0 ?g/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
The binding of PTEN antibody (4C11A11) to its target is sensitive to salt concentration. Please use the Biolegend recommended TBS/T buffer for Western blotting.
- Product Citations
-
- RRID
- AB_2561892 (BioLegend Cat. No. 655002)
Antigen Details
- Structure
- 403 amino acids, predicted molecular weight of 47 kD, and observed molecular weight of 54 kD. Contains a C2 tensin-like domain responsible for lipid binding and membrane localization.
- Distribution
-
Cytoplasm (unubiquitinated form); nucleus (mono-ubiquitinated form).
- Function
- PTEN functions as a tumor suppressor, modulating the PI3K-AKT-mTOR signaling pathway. It exerts phosphatase activity, dephosphorylating serine, threonine, and tyrosine phosphorylated proteins. In addition, dephosphorylates phospholipids, removing a phosphate from phosphatidylinositol-3,4,5-trisphosphate. The lipid phosphatase activity is required for its tumor suppressor function.
- Interaction
- Interacts with MAIG2, MAIG3, MAST1, MAST3, NEDD4, NDFIP1, NDFIP2, USP7, ROCK1, and XIAP. The unphosphorylated form interacts with AIP1, DLG1 and MAS.T2.
- Biology Area
- Angiogenesis, Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Signal Transduction, Transcription Factors
- Molecular Family
- Protein Kinases/Phosphatase
- Antigen References
-
1. Carracedo A, et al. 2011. Cancer Res. 71:629.
2. Jiang BH and Liu LZ. 2009. Adv Cancer Res. 102:19.
3. Mao JH, et al. 2008. Science 321:1499.
4. Weng LP, et al. 2001. Hum. Mol. Genet. 10:599.
5. Wrighton KH. 2011. Nat Rev Cancer. 11:154.
6. Wang X, et al. 2007. Cell 128:129. - Gene ID
- 5728 View all products for this Gene ID
- UniProt
- View information about PTEN on UniProt.org
