Product Details
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Partial human STAT3 recombinant protein (621-770 a.a.) expressed in E. coli.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
ICFC, ICC, IP, KO/KD-WB - Verified - Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 1.0 ?g per ml. For intracellular flow cytometric staining, the suggested use of this reagent is ≤ 0.06 ?g per million cells in 100 ?l volume. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/ml is recommended. For immunoprecipitation, the suggested use of this reagent is 2.0 - 10 μg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).
- Product Citations
-
- RRID
- AB_2861053 (BioLegend Cat. No. 678001) AB_2565913 (BioLegend Cat. No. 678002)
Antigen Details
- Structure
- 770 amino acids with a predicted molecular weight of 88.1 kD that contains a C-terminal SH2 domain responsible for homo- or herterodimerization.
- Distribution
-
Cytoplasm.
- Function
- Phosphorylated in response to cytokine signaling by receptor-associated kinases, translocates to the nucleus to act as a transcriptional activator, activates a wide variety of genes involved in cell growth and apoptosis, and is involved in gp130-related signaling.
- Interaction
- STAT3 forms a homodimer or a heterodimer with other STAT family members and interacts with IL31RA, NCOA1, PELP1, SIPAR, SOCS7, STATIP1, CAV2, DAPK3, EIF2AK2, PIAS3, and TMF1.
- Cell Type
- Embryonic Stem Cells, Mesenchymal Stem Cells, Neural Stem Cells
- Biology Area
- Cell Biology, Neuroscience, Neuroscience Cell Markers, Stem Cells, Synaptic Biology, Transcription Factors
- Molecular Family
- Nuclear Markers
- Antigen References
-
1. Lufei C, et al. 2003. EMBO J. 22:1325.
2. Deo D, et al. 2002. J. Biol. Chem. 277:21237.
3. Pfeffer L, et al. 1997. Science 276:1418.
4. Akira S, et al. 1994. Cell 77:63. - Gene ID
- 6774 View all products for this Gene ID
- UniProt
- View information about STAT3 on UniProt.org
