Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Partial human IRF9 recombinant protein (116-387 a.a.) expressed in E. coli.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
ChIP - Quality tested
WB, ICC - Verified - Recommended Usage
Each lot of this antibody is quality control tested by ChIP Assay. The suggested dilution for ChIP application is 1-50 to 1-200 by volume. For Western blotting, the suggested use of this reagent is 0.4 - 2.5 ?g per mL. It is recommended that the reagent to be titrated for optimal performance before each experiment.
- Application Notes
This clone does not react with mouse (in-house tested). A non-specific band was observed (around 37 KD) when this antibody was tested on Raw264.7 cells
- RRID
- AB_2721591 (BioLegend Cat. No. 660703) AB_2721592 (BioLegend Cat. No. 660704)
Antigen Details
- Structure
- 393 amino acids, predicted molecular weight of 44 kD; contains a IRF tryptophan pentad repeat responsible for DNA binding.
- Distribution
-
Cytoplasm, phosphorylated form translocated to the nucleus.
- Function
- IRF9 is a transcription factor that mediates interferon signaling and activates the expression of interferon inducible genes.
- Interaction
- Forms the interferon-stimulated gene factor 3 complex (ISGF3) with Signal Transducer and Activator of Transcription 1 (STAT1) and STAT2 in response to type I IFN stimulation.
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Signal Transduction, Transcription Factors
- Molecular Family
- Nuclear Markers
- Antigen References
-
1. Fink K and Grandvaux N, 2013. JAKSTAT. 2:e27521.
2. Tsuno T, et al. 2009. J. Immunother. 32:803.
3. Thibault DL, et al. 2008. J. Clin. Invest. 118:1417.
4. Kraus TA, et al. 2003. J. Biol. Chem. 278:13033.
5. Xiao W, et al. 2001. J. Biol. Chem. 276:23275.
6. Veals SA, et al. 1992. Mol. Cell. Biol. 12:3315. - Gene ID
- 10379 View all products for this Gene ID
- UniProt
- View information about IRF9 on UniProt.org
