Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Endogenous protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
IHC-P - Verified
FC - Reported in the literature, not verified in house - Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.5 ?g per ml. For immunohistochemistry, a concentration range of 0.5 - 5 ?g per ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
The observed molecular weight of this target is ~500 kD, which is considerably different than the predicted MW of 1519 kD. Similar observed molecular weights were confirmed using control antibodies from other vendors and have been reported by other vendors.
- Application References
(PubMed link indicates BioLegend citation) -
- Goodell CA, et al. 2009. J. Ovarian Res. 2:8. (FC)
- RRID
- AB_2629540 (BioLegend Cat. No. 666904)
Antigen Details
- Structure
- CA125 is a 250 kD+ protein.
- Biology Area
- Cancer Biomarkers, Cell Biology
- Antigen References
-
1. Yin BW, et al. 2001. J. Biol. Chem. 276:27371.
2. Duraisamy S, et al. 2006. Gene. 373:28.
3. Bast RC Jr, et al. 1998. Int. J. Biol. Markers 13:179. - Gene ID
- 94025 View all products for this Gene ID 73732 View all products for this Gene ID
- UniProt
- View information about CA125 on UniProt.org