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Purified anti-CA125 Antibody

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Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Endogenous protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P - Verified
FC - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.5 ?g per ml. For immunohistochemistry, a concentration range of 0.5 - 5 ?g per ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The observed molecular weight of this target is ~500 kD, which is considerably different than the predicted MW of 1519 kD. Similar observed molecular weights were confirmed using control antibodies from other vendors and have been reported by other vendors.

Application References

(PubMed link indicates BioLegend citation)
  1. Goodell CA, et al. 2009. J. Ovarian Res. 2:8. (FC)
RRID
AB_2629540 (BioLegend Cat. No. 666904)

Antigen Details

Structure
CA125 is a 250 kD+ protein.
Biology Area
Cancer Biomarkers, Cell Biology
Antigen References

1. Yin BW, et al. 2001. J. Biol. Chem. 276:27371.
2. Duraisamy S, et al. 2006. Gene. 373:28.
3. Bast RC Jr, et al. 1998. Int. J. Biol. Markers 13:179.

Gene ID
94025 View all products for this Gene ID 73732 View all products for this Gene ID
UniProt
View information about CA125 on UniProt.org
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