Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human TOX recombinant protein (66-301 a.a.) expressed in E. coli.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 594 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor? 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses- Application Notes
The immunogen used to generate clone 6E6D03 is the N-terminus of Tox1, which is highly conserved among Tox family member proteins. Hence, this clone may recognize multiple Tox family members. Predicted MW for Tox family members: Tox 1 is 57.5 kD, Tox 2 is 49, 52, 53, 55 kD, Tox 3 is 63 kD and Tox 4 is 64, 66 kD.
- RRID
- AB_2566786 (BioLegend Cat. No. 682604)
Antigen Details
- Structure
- 562 amino acids with a predicted molecular weight of 57.5 kD. Contains a high mobility group (HMG) box domain responsible for DNA binding.
- Distribution
-
Nucleus.
- Function
- TOX is a transcription factor required for development of various T lymphocyte subsets.
- Interaction
- TOX interacts with ZDHHC17.
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Transcription Factors
- Molecular Family
- Nuclear Markers
- Antigen References
-
1. Spits H. 2015. Nat. Immunol. 16:594.
2. Seehus CR, et al. 2015. Nat. Immunol. 16:599.
3. Huang Y, et al. 2015. Blood 125:1435.
4. Aliahmad P, et al. 2012. Curr. Opin. Immunol. 24:173.
6. Di Santo JP. 2010. Nat. Immunol. 11:885.
7. Aliahmad P, et al. 2004. J. Exp. Med. 199:1089. - Gene ID
- 9760 View all products for this Gene ID
- UniProt
- View information about TOX on UniProt.org
