Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Partial TCF1 recombinant protein (116-334 aa)
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 647 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICFC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear? Transcription Factor Staining Protocol.?For flow cytometric staining, the suggested use of this reagent is 5 ?l per 106 cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses- Excitation Laser
- Red Laser (633 nm)
- Application Notes
NOTE: For flow cytometric staining with this clone, True-Nuclear? Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended.
- Product Citations
-
- RRID
- AB_2566619 (BioLegend Cat. No. 655203) AB_2566620 (BioLegend Cat. No. 655204)
Antigen Details
- Structure
- 384 amino acids, predicted molecular weight of 42 kD. Contains a HMG box DNA binding domain and a CTNNB1 (β-catenin) binding domain.
- Distribution
-
Nucleus
- Function
- TCF1 is a transcription factor, involved in the canonical Wingless/Integration 1 (Wnt) signaling pathway. TCF1 is essential for survival of CD4+CD8+ double positive thymocytes and differentiation of T cells in the periphery.
- Interaction
- TCF1 interacts with CTNNB1, TLE1, TLE2, TLE3, TLE4, and AES.
- Cell Type
- Tregs
- Biology Area
- Cell Biology, Immunology, Transcription Factors
- Molecular Family
- Nuclear Markers, TCRs
- Antigen References
-
1. Mao CD, et al. 2011. Crit. Rev. Eukaryot. Gene Expr. 21:207.
2. Germar K, et al. 2011. P. Natl. Acad. Sci. USA 108:20060.
3. Wang R, et al. 2011. J. Immunol. 187:5964.
4. Weber BN, et al. 2011. Nature 476:63.
5. Jeannet G, et al. 2010. P. Natl. Acad. Sci. USA 107:9777.
6. Staal FJ, et al. 1999. Int. Immunol. 11:317. - Gene ID
- 6932 View all products for this Gene ID
- UniProt
- View information about TCF1 on UniProt.org
