Product Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Partial mouse CD204 recombinant protein (79-458 a.a.) expressed in 293E cells.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 1.0 ?g per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
CD204 is polymorphic and clone 7G5C33 may not react with all mouse strains. In house testing with this clone showed no reactivity to C57BL/6 mouse spleen samples.
The predicted molecular weight of CD204 is 50 kD, the observed molecular weight when clone 7G5C33 is used for western blot is ~70 kD. This is likely due to glycosylation of CD204 at multiple sites.- RRID
- AB_2565623 (BioLegend Cat. No. 676202)
Antigen Details
- Structure
- 485 amino acids with a predicted molecular weight of 50 kD and is a single-pass type II membrane protein.
- Distribution
-
Plasma membrane, low-density lipoprotein particle, and endocytic vesicle emmbrane.
- Function
- CD204, a trimeric type II integral membrane glycoprotein, is predominantly expressed on macrophages and dendritic cells. CD204 plays important roles in lipid metabolism, host defense, and inflammatory responses.
- Interaction
- CD204 interacts with HSPA1A.
- Biology Area
- Cell Biology, Immunology, Innate Immunity
- Molecular Family
- CD Molecules
- Antigen References
-
1. Kelley JL, et al. 2014. Crit. Rev. Immunol. 34:241.
2. Saito Y, et al. 2014. J. Clin. Exp. Hematop. 54:59.
3. Jozefowski S, et al. 2014. Innate Immun. 20:826.
4. Haasken S, et al. 2013. J. Immunol. 191:1055.
5. Yu H, et al. 2012. Biochim. Biophys. Acta. 7:1192.
6. Yu X, et al. 2012. Oncoimmunology 5:770. - Gene ID
- 20288 View all products for this Gene ID
- UniProt
- View information about CD204 on UniProt.org
