Product Details
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Partial human DDX17 recombinant protein (1-107 a.a.)
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
ICC, IP - Verified - Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 0.5 ?g per ml.?For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/ml is recommended. For immunoprecipitation, the suggested use of this reagent is 5.0 - 20 μg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
This antibody, clone 7G6B09, detects both human DDX17 isoform-1 (p82) and isoform-2 (p72) by Western blotting. Clone 1B5B09 detects only human DDX17 isoform-1 (p82) by Western blotting.
- RRID
- AB_2562551 (BioLegend Cat. No. 657302)
Antigen Details
- Structure
- 729 amino acids, predicted molecular weight of 80 kD. Contains a helicase domain, an ATP-binding domain, and a transactivation domain.
- Distribution
-
Nucleus
- Function
- DDX17 is a member of the DEAD box superfamily of RNA helicases, involved in RNA metabolism and transcriptional regulation.
- Interaction
- DDX17 interacts with DDX5, RNA polymerase II, CBP, p300, PCAF, NCOA1, NCOA2, NCOA3, ESR1, TP53, and HDAC1.
- Biology Area
- Cell Biology, Cell Cycle/DNA Replication, Signal Transduction, Transcription Factors
- Molecular Family
- Nuclear Markers
- Antigen References
-
1. Janknecht R. 2010. Am. J. Transl. Res. 2:223.
2. Wortham NC, et al. 2009. Oncogene. 28:4053.
3. Shin S, et al. 2007. Cancer Res. 67:7572.
4. Caretti G, et al. 2007. Cell Cycle 6:1172.
5. H?nig A, et al. 2002. Mol. Cell Biol. 22:5698.
6. Lamm GM, et al. 1996. Nucleic Acids Res. 24:3739. - Gene ID
- 10521 View all products for this Gene ID
- UniProt
- View information about DDX17 on UniProt.org
