Product Details
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Partial human STAT5A recombinant protein (627-794 a.a.) expressed in E. coli.
- Formulation
- This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- Affinity purified.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- Upon receipt, store undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
KO/KD-WB, ICC, IP - Verified - Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 2.0 ?g per ml. For immunocytochemistry, the suggested use of this reagent is 1.0 - 5.0 ?g per ml.?For immunoprecipitation, the suggested use of this reagent is 2.0 - 10.0 μg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).
- Product Citations
-
- RRID
- AB_2563469 (BioLegend Cat. No. 660202)
Antigen Details
- Structure
- 794 amino acids, predicted molecular weight of 91 kD; contains a SH2 domains responsible for homodimerization or heterodimerization.
- Distribution
-
Cytoplasm; phosphorylated form translocates to the nucleus.
- Function
- STAT5 is a transcription regulator and is involved in cell differentiation, proliferation, and survival, in response to several cytokines, such as interleukins, erythropoietin, growth hormone, thrombopoietin, and prolactin.
- Interaction
- Forms a homodimer or a heterodimer with other family members. Interacts with NCOA1, SOCS7, CBP, p300, SMRT and ERBB4.
- Cell Type
- B cells, Tregs
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Immunology, Signal Transduction, Transcription Factors
- Molecular Family
- Nuclear Markers
- Antigen References
-
1. Bell BD, et al. 2013. Nat. Immunol. 14:364.
2. Cholez E, et al. 2012. Leukemia. 26:2390.
3. Heltemes-Harris LM, et al. 2012. Curr. Opin. Immunol. 24:146.
4. Heltemes-Harris LM, et al. 2011. Ann. N Y Acad. Sci. 1217:18.
5. Malin S, et al. 2010. Curr. Opin. Immunol. 22:168.
6. Xu J, et al. 2009. J. Neurosci. 29:2022. - Gene ID
- 6776 View all products for this Gene ID
- UniProt
- View information about STAT5 on UniProt.org
