Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Partial human SWAP70 recombinant protein (89-585 aa).
- Formulation
- This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.05% sodium azide.
- Preparation
- Affinity purified.
- Storage & Handling
- Upon receipt, store undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
IP - Verified - Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1-0.5 ?g per ml. For immunoprecipitation, the suggested use of this reagent is 10-30 μg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
- AB_2561890 (BioLegend Cat. No. 654702)
Antigen Details
- Structure
- Contains a PH domain, which is required for binding of phosphatidylinositol 3,4,5-trisphosphate, a coil-coil domain, and a domain weakly homologous to DH domains. Theoretical molecular weight is 69 kD.
- Distribution
- SWAP70 is localized to cytoplasm in resting B-cells, recruited to plasma membrane, and translocated to the nucleus upon B-cell activation.
- Function
- SWAP70 is a guanine nucleotide exchange factor (GEF) which binds phosphatidylinositol trisphosphate and transduces signals from tyrosine kinase receptors to RAC. SWAP70 is required for membrane ruffling and lamellipodia formation during cytoskeletal F-actin rearrangement.
- Interaction
- Interacts with cytoskeletal F-actin and Rac to regulate actin remodeling. Associates with NPM1, NCL, and PARP1 in switch-associated protein complex.
- Cell Type
- B cells
- Biology Area
- Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology, Signal Transduction
- Antigen References
-
1. Masat L, et al. 2000. Proc. Natl. Acad. Sci. USA 97:2180.
2. Hilpel? P, et al. 2003. Mol. Biol. Cell. 14:3242.
3. Ihara S, et al. 2006. J. Cell Sci. 119:500.
4. Shinohara M, et al. 2002. Nature 416:759.
5. Pearce G, et al. 2006. Nat. Immunol. 7:827. - Gene ID
- 23075 View all products for this Gene ID
- UniProt
- View information about SWAP70 on UniProt.org
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