Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant Human CCL23, amino acids Arg46-Asn120 (Accession# P55773.3) was expressed in E. coli.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. May be sub-aliquoted and stored at -20°C to avoid multiple freeze thaw cycles.
- Application
-
WB - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 ?g/ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Please note that for western blotting, this antibody works only under non-reducing conditions. Please make sure there is no DTT or beta-Mercaptoethanol in your cell lysate or sample buffer. This antibody is only tested on recombinant proteins. It may not be able to detect endogenous protein from cell or tissue lysates by western blotting where the expression levels may be too low.
- RRID
- AB_2616703 (BioLegend Cat. No. 684002)
Antigen Details
- Structure
- 120 amino acids with a predicted molecular weight of approximately 13kD.
- Distribution
-
Monocytes, macrophages, dendritic cells, mucosal epithelial cells, and eosinophils.
- Function
- CCL23 attracts monocytes, dendritic cells, and resting lymphocytes. CCL23 induces endothelial cell migration and angiogenesis. CCL23 inhibits colony formation of bone marrow-derived cells. IL-4 and IL-13 induce CCL23 in peripheral blood monocytes, and GM-CSF synergizes with IL-4 to induce CCL23. GM-CSF and IL-5 induce CCL23 secretion from eosinophils.
- Ligand/Receptor
- CCR1.
- Cell Type
- Monocytes, Macrophages, Dendritic cells, Epithelial cells, Eosinophils
- Biology Area
- Angiogenesis, Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology, Innate Immunity, Signal Transduction
- Molecular Family
- Cytokines/Chemokines
- Antigen References
-
1. Patel VP, et al. 1997. J. Exp. Med. 185:1163.
2. Macphee CH, et al. 1998. J. Immunol. 161:6273.
3. Hwang J, et al. 2005. Cytokine. 30:254.
4. Berahovich RD, et al. 2005. J. Immunol. 174:7341.
5. Novak H, et al. 2007. J. Immunol. 178:4335.
6. Rioja I, et al. 2008. Arthritis Rheum. 58:2257.
7. Poposki JA, et al. 2011. J. Allergy Clin. Immunol. 128:73.
8. Starr AE, et al. 2012. J. Biol. Chem. 287:5848. - Gene ID
- 6368 View all products for this Gene ID
- UniProt
- View information about CCL23 on UniProt.org
