Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant human IGFBP4
- Formulation
- 0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/?g of the protein (<0.001 ng/?g of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.
- Application
-
Blocking - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by blocking activity. The ND50 is 4 - 12 ?g/mL. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
- AB_2687181 (BioLegend Cat. No. 697403) AB_2687182 (BioLegend Cat. No. 697404)
Antigen Details
- Distribution
-
It is expressed in a variety of tissues and cells such as embryonic and postnatal brain, human osteoblast-like cells, skin fibroblasts, endothelial cells, epithelial cells, intestinal epithelium, and in a variety of tumor cells.
- Function
- IGFBP-4 inhibits the action of IGFI and II by preventing their binding to their receptors. The binding affinity of IGFBP-4 for IGFs is regulated by proteolysis through PAPP-A.
- Ligand/Receptor
- IGF-I, IGF-II
- Biology Area
- Angiogenesis, Cancer Biomarkers, Cell Biology, Cell Proliferation and Viability
- Antigen References
-
1. Shimasaki S, et al. 1990. Molecular Endocrinol. 4:1451.
2. Boldt HB, et al. 2001. Biochem. J. 358:359.
3. Bunn RC and Fowlkes JL. 2003. Trends Endocrinol. Metab. 14:176.
4. Ning Y, et al. 2008. Mol. Endocrinol. 22:1213.
5. Moreno MJ, et al. 2013. Neoplasia. 5:554.
6. Contois LW, et al. 2012. J. Biol. Chem. 287:1779.
7. Shi Z, et al. 2013. Am. J. Physiol. Gastrointest. Liver Physiol. 305:G74. - Gene ID
- 3487 View all products for this Gene ID
- UniProt
- View information about IGFBP-4 on UniProt.org
