Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 5% HPCD
- Preparation
- The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
- Application
-
ELISA Detection - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by ELISA assay. For ELISA Detection applications, a concentration range of 1 - 4 ?g/mL is recommended. To obtain a linear standard curve, serial dilutions of BDNF recombinant protein ranging from 156.3 to 10,000 pg/mL is recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
The biotin A15062F antibody is useful as the detection antibody in a sandwich ELISA assay, when used in conjunction with purified A15062G antibody (Cat. No. 536702) as the detection antibody and Recombinant Human BDNF (ELISA Std.) (Cat. No. 759709).
Although not tested, additional species reactivity for rat, donkey, equine, porcine, and monkey are predicted based on 100% amino acid sequence conservation for this target across listed species.- RRID
- AB_2783343 (BioLegend Cat. No. 536804)
Antigen Details
- Structure
- Noncovalently linked homodimer.
- Distribution
-
Ubiquitous with high levels in peripheral tissues including prostate, placenta, pancreas, heart, kidney, pituitary gland, lung, and testis.
- Function
- Supports the survival of sensory and sympathetic neurons, and also dopaminergic midbrain neurons.
- Interaction
- Sensory neurons, sympathetic neurons, midbrain dopaminergic neurons
- Ligand/Receptor
- TrkB
- Antigen References
-
- Baloh RH, et al. 1998. Neuron. 21:1291.
- Masure S, et al. 1999. Eur. J. Biochem. 266:892-902.
- Gardell LR, et al. 2003. Nat. Med. 9(11):1383-9.
- Honma Y, et al. 2002. Neuron. 35:267-82.
- Banerjee A, et al. 2012. PLoS One 7(11):e50098.
- Wong LE, et al. 2015. Proc. Natl. Acad. Sci. USA 112:6170-5.
- Zhang M, et al. 2016. Oncotarget. 7:3267-82.
- Gene ID
- 627 View all products for this Gene ID
- UniProt
- View information about BDNF on UniProt.org
