Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant human Activin A.
- Formulation
- 0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/?g of the protein (<0.001 ng/?g of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.
- Application
-
Neut - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by neutralizing the inhibition of proliferation induced by human recombinant Activin A (30 ng/ml) on mouse plasmacytoma cell line MPC 11. ND50 range:? 0.35 – 1.05 ?g/mL. It is recommended that the reagent be titrated for optimal performance for each application.
- Product Citations
-
- RRID
- AB_2632840 (BioLegend Cat. No. 693603) AB_2632841 (BioLegend Cat. No. 693604)
Antigen Details
- Structure
- Homodimer.
- Distribution
-
BM-derived stromal fibroblasts, aortic smooth muscle cells, pulmonary epithelium, and CD4 T cells.
- Function
- Activin A plays a key role in embryogenesis, neointimal formation after arterial injury, early stages of chondrogenesis, osteogenesis, and hemopoiesis. Activin A drives TH9 differentiation in vitro. It is induced by Angiotensin II and alpha Thrombin and regulated by Follistatin. Activin A is induced by inflammatory cytokines and inhibited by glucocorticoids.
- Interaction
- The receptors for Activin A are expressed by many stromal cells, among them fibroblasts, hepatocytes, myometral cells, vascular endothelial cells, and neuronal progenitor cells.
- Ligand/Receptor
- Type I receptors ALK4 and ALK7, type II receptors ActRIIA and ActRIIB.
- Cell Type
- Fibroblasts, Epithelial cells, T cells
- Biology Area
- Cell Biology
- Antigen References
-
1. Schwall RH, et al. 1988. Mol. Endocrinol. 2:1237.
2. Hashimoto M, et al. 1992. J. Biol. Chem. 267:4999.
3. Shav-Tal Y, Zipori D. 2002. Stem Cells 20:493.
4. Dohi T, et al. 2005. Gastroenterology 128:411.
5. Tsuchida K, et al. 2008. Endocr. J. 55:11.
6. Djouad F, et al. 2010. Stem Cell Research 1:11.
7. Sako D, et al. 2010. J. Biol. Chem. 285:21037.
8. Jones CP, et al. 2012. J. Allergy Clin. Immunol. 129:1000. - Gene ID
- 3624 View all products for this Gene ID
- UniProt
- View information about Activin A on UniProt.org
