Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant human A100A8/A9
- Formulation
- 0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/?g of the protein (<0.001 ng/?g of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.
- Application
-
Block - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by blocking activity. The ND50 is 2 - 8 ?g/mL. ?It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
- AB_2687183 (BioLegend Cat. No. 697503) AB_2687184 (BioLegend Cat. No. 697504)
Antigen Details
- Structure
- Non-covalent heterodimer.
- Distribution
-
Neutrophils, monocytes, macrophages, gingival keratinocytes, granulocytes, osteoclasts, fibroblasts, and microvascular endothelial cells.
- Function
- Pro-inflammatory molecule that possesses antimicrobial properties. Is up-regulated in neutrophils and monocytes at the sites of inflammation.
- Interaction
- Astrocytes, neutrophils, and monocytes.
- Ligand/Receptor
- RAGE, TLR4
- Cell Type
- Neutrophils, Monocytes, Macrophages, Granulocytes, Osteoclasts, Fibroblasts, Endothelial cells
- Biology Area
- Cell Biology, Neuroscience, Stem Cells, Synaptic Biology
- Molecular Family
- Growth Factors, Cytokines/Chemokines
- Antigen References
-
1. Sampson B, et al. 2002. Lancet. 360:1742-5.
2. Vogl T, et al. 2007. Nat. Med. 13:1042-9.
3. Semprini S, et al. 2002. Hum. Genet. 111:310-3.
4. Corbin BD, et al. 2008. Science 319:962-5.
5. Gopal R, et al. 2013. Am. J. Respir. Crit. Care Med. 188:1137-46.
6. Siegenthaler G, et al. 1997. J. Biol. Chem. 14:9371-7.
7. Ryckman C, et al. 2003. J. Immunol. 170:3233-42.
8. Ryu MJ, et al. 2012. J. Biol. Chem. 287:22948-58.
9. Ehrchen JM, et al. 2009. J. Leuko. Biol. 3:557-66.
10. Wang J, et al. 2015. FASEB J. 29(1):250-62.
11. Champaiboon C, et al. 2009. J. Biol. Chem. 284:7078. - Gene ID
- 6279 View all products for this Gene ID
- UniProt
- View information about S100A8/A9 Heterodimer on UniProt.org
