Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
ELISA Capture - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by ELISA assay. For ELISA Capture applications, a concentration range of 1 - 4 ?g/mL is recommended. To obtain a linear standard curve, serial dilutions of VEGF recombinant protein ranging from 23.4 to 1,500 pg/mL is recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
The purified A15136H antibody is useful as the capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated Poly5225 antibody (Cat. No. 522503) as the detection antibody and Recombinant Human VEGF (ELISA Std.) (Cat. No. 560309)
- RRID
- AB_2783345 (BioLegend Cat. No. 537002)
Antigen Details
- Structure
- Homodimer
- Distribution
-
Widely expressed
- Function
- VEGFA is a key player in vasculogenesis, the formation of blood vessels from progenitor cells, as well as angiogenesis. The expression of the VEGFA gene is upregulated via hypoxia, estrogen, and NF-κB pathways. In addition VEGF is upregulated by PDGF-BB, P1GF, TGFβ1, IGF1, FGFs, HGF, TNFα, and IL-1. VEGFA induces proliferation and cell migration in endothelial cells and plays important roles during wound healing. Also, VEGF regulates hematopoietic stem cell survival.
- Interaction
- VEGFA interacts with vascular endothelial cells and monocytes/macrophages, which express VEGFR1. This interaction induces proliferation of endothelial cells and stimulates migration of monocytes/macrophages
- Ligand/Receptor
- Vascular endothelial growth factor (VEGF)-A binds and activates two tyrosine kinase receptors, VEGFR1 (Flt-1) and VEGFR2 (KDR/Flk-1)
- Molecular Family
- Cytokines/Chemokines
- Antigen References
-
- Conn G, et al. 1990. Proc. Natl. Acad. Sci USA 87:1323.
- Gerber H, et al. 2002. Nature 417:954.
- Shibuya M. 2006. J. Biochem. Mol. Biol. 39:469.
- Shibuya M. 2008. BMB Rep. 41:278.
- Monaghan-Benson E, et al. 2010. The American Journal of Pathology 177:2091.
- Koch S and Claesson-Welsh L. 2012. Cold Spring Harb Perspect Med. 2:a006502.
- Gene ID
- 7422 View all products for this Gene ID
- UniProt
- View information about VEGF on UniProt.org
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