Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human STAT6 peptide phosphorylated at Tyr 641
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
ICFC - Verified - Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 ?g/ml. For intracellular flow cytometric staining, the suggested use of this reagent is ≤0.03 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Human STAT6 has three isoforms; the molecular weights are 94, 82 and 74kD. The immunogen (phosphorylated peptide) is shared by these three isoforms. This antibody recognizes STAT6 Phospho (Tyr641) in all three isoforms. The predominant band detected is at 94 kD.
Clone A15137E does not react with mouse.
This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).- Product Citations
-
- RRID
- AB_2616820 (BioLegend Cat. No. 686002)
Antigen Details
- Structure
- 847 amino acids with a predicted molecular weight of 94 kD. Contains a SH2 domain responsible for phosphor-Tyrosine binding and dimerization.
- Distribution
-
Cytoplasm; translocates to the nucleus when phosphorylated.
- Function
- STAT6 is a transcription factor, mediating IL-4 and IL-13 signaling. While STAT6 is widely expressed in human tissues, it exhibits elevated expression in peripheral blood lymphocytes, colon, intestine, ovary, prostate, thymus, spleen, kidney, liver, lung, and placenta. Following phosphorylation by Janus kinases, STAT6 translocates to the nucleus where it regulates gene transcription.
- Interaction
- STAT6 interacts with CBP/p300 and NCOA1. Forms a homodimer or a heterodimer with other STAT family members.
- Cell Type
- B cells
- Biology Area
- Cell Biology, Immunology, Signal Transduction
- Molecular Family
- Nuclear Markers, Phospho-Proteins
- Antigen References
-
1. Goenka S, et al. 2011. Immunol. Res. 50:87.
2. Wurster AL, et al. 2000. Oncogene 19:2577.
3. Akira S. 1999. Stem Cells 17:138.
4. Zamorano J, et al. 2005. J. Immunol. 174:2843.
5. David M, et al. 2001. Oncogene 20:6660.
6. Takeda K, et al. 1996. Nature 380:627. - Gene ID
- 6778 View all products for this Gene ID
- UniProt
- View information about STAT6 Phospho Tyr641 on UniProt.org
