Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant Human TIGIT.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with APC/Cyanine7 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?L per million cells in 100 ?L staining volume or 5 ?L per 100 ?L of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
- Red Laser (633 nm)
- Application Notes
This clone can suppress anti-CD3 induced T cell proliferation in vitro based on in-house testing.
This clone has been tested in-house and determined to not be suitable for applications in immunohistochemistry of paraffin-embedded tissue sections (IHC-P).
Additional reported applications (for the relevant formats) include: Blocking1.- Application References
(PubMed link indicates BioLegend citation) -
- Stamm H, et al. 2018. Oncogene. Pubmed
- Product Citations
-
- RRID
- AB_2876700 (BioLegend Cat. No. 372733) AB_2876700 (BioLegend Cat. No. 372734)
Antigen Details
- Structure
- 26kD; type I transmembrane protein, Ig-like V-type domain, ITIM motif.
- Distribution
-
Activated T cells, Regulatory T cells (Treg), Follicular Helper T cells (TFH), NK cells.
- Function
- Cell signaling, negative regulation of T cells, T cell tolerance, T cell anergy.
- Ligand/Receptor
- CD155 (PVR), CD112 (PVRL2, NECTIN-2).
- Cell Type
- NK cells, T cells, Tfh, Tregs
- Biology Area
- Cell Adhesion, Cell Biology, Immunology, Inhibitory Molecules, Signal Transduction
- Molecular Family
- Adhesion Molecules, Immune Checkpoint Receptors
- Antigen References
-
1. Stanietsky N, et al. 2009. Proc. Natl. Acad. Sci. 106:17858.
2. Yu X, et al. 2009. Nat. Immunol. 10:48.
3. Johnston R, et al. 2014. Cancer Cell. 26:923. - Gene ID
- 201633 View all products for this Gene ID
- UniProt
- View information about TIGIT on UniProt.org
