Product Details
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human STAT1 peptide phosphorylated at Serine 727.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.05 - 0.25 ?g per ml (1:2000 - 1:5000 dilution). It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
This antibody is specific for STAT1 isoform a and is not predicted to recognize the ? isoform due to the absence of the region corresponding to the immunizing peptide.
This clone displayed a stronger affinity for STAT1a in parallel western blot testing with BioLegend’s anti-STAT1 antibody (clone 10C4B40).
U3A cells were selected for antibody specificity testing because the cell line does not express STAT11.
This clone was derived from a human STAT1 peptide phosphorylated at Serine 727 immunogen, but tested negative for phospho-recognition while displaying strong pan-reactivity. Supplemental data is provided confirming the differences in phosphorylation of STAT1 Serine 727 in HeLa cells treated with or without nocodazole.- Application References
(PubMed link indicates BioLegend citation) -
- Muller M, et al. 1993. EMBO J. 12:4221
- Product Citations
-
- RRID
- AB_2749867 (BioLegend Cat. No. 603701) AB_2749868 (BioLegend Cat. No. 603702)
Antigen Details
- Structure
- STAT1α is a 750 amino acid protein with a predicted molecular weight of 87 kD; contains an SH2 domain responsible for homodimerization or heterodimerization.
- Distribution
-
Ubiquitously expressed; cytosolic and nuclear distribution
- Function
- Transcription activator/Cytokine Signaling
- Interaction
- Forms a homodimer or heterodimers with other family members. Interacts with FAK, MCM3, MCM5, TRADD, BRCA1, KIT, IL-27R, IL-2Rβ, IL-2Rγ, IFNαβR, and c-Src.
- Biology Area
- Cell Biology, Signal Transduction, Transcription Factors
- Molecular Family
- Nuclear Markers
- Antigen References
-
- Durbin JE, et al. 1996. Cell 8:443.
- Darnell JE Jr, et al. 1994. Science 264:1415.
- Chen X, et al. 1998. Cell 93:827.
- Ramana CV, et al. 2000. Oncogene 19:2619.
- Gene ID
- 6772 View all products for this Gene ID
- UniProt
- View information about STAT1 on UniProt.org
