Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant human CCL21 (6CKine)
- Formulation
- 0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is < 0.01 EU/?g of the protein (< 0.001 ng/?g of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.
- Application
-
Neut - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by neutralizing the chemotaxis induced by recombinant human CCL21 (6CKine) (Cat. No. 582202) at 6 ng/mL on mouse Baf3- hCCR7 transfectants. ND50 range: 0.06?- 0.6??g/mL. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
- AB_2922638 (BioLegend Cat. No. 604353) AB_2922638 (BioLegend Cat. No. 604354)
Antigen Details
- Structure
- Chemokine
- Distribution
-
CCL21 is highly expressed in HEV of lymph nodes and Peyer's patches. Also, it is expressed in the T cell zones of lymph nodes, in Peyer's patches by reticular cells, and in spleen by stromal cells. CCL21 is expressed by endothelial cells in lymphatic venules.
- Function
- CCL21 chemoattracts na?ve T cells and maturing dendritic cells (DCs) into T zones of secondary lymphoid organs. CCL21 and CCL19 chemoattract antigen-engaged B cells to the boundary of B and T zones to interact with T-helper cells. CCL19 and CCL21 inhibit apoptosis in DCs.
- Interaction
- Thymocytes, activated T cells, na?ve CD4 and CD8, mature DCs, fibroblast-like synoviocytes of patients with rheumatoid arthritis (RA)
- Ligand/Receptor
- CCR7 (CD197)
- Cell Type
- B cells, Dendritic cells, Tregs
- Biology Area
- Angiogenesis, Cell Biology, Immunology
- Molecular Family
- Cytokines/Chemokines
- Antigen References
-
- Hedrick JA and Zlotnik A. 1997. J. Immunol. 159:1589.
- Reif K, et al. 2002. Nature 416:94.
- Ziegler E, et al. 2007. J. Immunol. 179:6485.
- Pickens SR, et al. 2012. Arthritis Rheum. 10.1002.
- Gene ID
- 6366 View all products for this Gene ID
- UniProt
- View information about CCL21 on UniProt.org
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