Product Details
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Recombinant
- Host Species
- Mouse
- Immunogen
- Synthetic peptide corresponding to STAT3 phosphorylated at tyrosine 705
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
ICC, ICFC - Verified - Recommended Usage
Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.5 - 1.0??g/mL. For immunocytochemistry, a concentration range of 5.0 - 10.0 μg/mL is recommended. For flow cytometric staining using our True-Phos? Perm Buffer in Cell Suspensions Protocol, the suggested use of this reagent is ≤ 0.06??g per million cells in 100 ?L volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
This clone shows high pan-reactivity to STAT3 when used for WB. BioLegend clone 13A3-1 is more suitable for phospho-specific detection of STAT3.
This recombinant antibody clone is derived from clone A16002B.- RRID
- AB_2894516 (BioLegend Cat. No. 690451) AB_2894516 (BioLegend Cat. No. 690452)
Antigen Details
- Structure
- STAT3 is a 770 amino acid protein of 88 kD. It consists of a DNA binding domain, a SH2 domain, a regulatory tyrosine responsible for binding of SH2 domain, and a C-terminal transactivation domain.
- Distribution
-
Ubiquitous
- Function
- STAT3 is tyrosine phosphorylated by receptor kinases in response to a variety of cytokines and growth factors. It forms homo- or heterodimer with STAT1 when tyrosine is phosphorylated, and then translocates to nucleus, acting as a transcription regulator. It is also essential for the differentiation of TH17 cells, which is involved in autoimmune diseases. The small GTPase Rac1 binds and regulates activity of STAT3.
- Ligand/Receptor
- When activated, STAT3 dimerizes and translocates to the nucleus where it regulates gene expression
- Cell Type
- Embryonic Stem Cells
- Biology Area
- Cell Biology, Neuroscience, Neuroscience Cell Markers, Signal Transduction, Stem Cells
- Molecular Family
- Nuclear Markers, Phospho-Proteins
- Antigen References
-
- Akira S, et al. 1994. Cell. 77:63.
- Zhang X, et al. 1995. Science. 267:1990.
- Sanchez-Margalet V, et al. 2001. Cell Immunol. 211:30.
- Simon A, et al. 2000. Science. 290:144.
- Hoey T, et al. 1999. Adv Immunol. 71:145.
- Gene ID
- 6774 View all products for this Gene ID
- UniProt
- View information about STAT3 on UniProt.org
