Product Details
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human S6 peptide.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
ICFC, KO/KD-WB, IHC-P - Verified - Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 ?g per ml.?For?intracellular flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per million cells in 100 ?l volume. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0?- 5.0??g/ml is suggested.It is recommended that the reagent be titrated for optimal performance for each application.
- Product Citations
-
- RRID
- AB_2629833 (BioLegend Cat. No. 691802)
Antigen Details
- Structure
- 249 amino acids with a predicted molecular weight of 28.7kD.
- Distribution
-
Cytoplasm and nucleus.
- Function
- Ribosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest.
- Interaction
- NCBP1, PASK.
- Biology Area
- Cell Biology, Signal Transduction
- Antigen References
-
1. Kressler A, et al. 2010. Biochim. Biophys. Acta. 1803:673.
2. Biever A, et al. 2015. Front. Mol. Neurosci. 8:75.
3. Ventimiglia FA, Wool IG. 1974. Proc. Natl. Acad. Sci. USA 71:350.
4. Lastick SM, et al. 1977. Mol. Gen. Genet. 152:223.
5. Ruvinsky I, et al. 2005. Genes Dev. 19:2199.
6. Meyuhas O. 2008. Int. Rev. Cell. Mol. Biol. 268:1.
7. Meyuhas O. 2015. Int. Rev. Cell. Mol. Biol. 320:41. - Gene ID
- NA
- UniProt
- View information about RPS6 on UniProt.org
