Product Details
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human p38 MAPK peptide phosphorylated at Thr 180 and Tyr182
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICFC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?L per million cells in 100 ?L staining volume or 5 ?L per 100 ?L of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
- Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
29kD isoform of p38a may show up with longer exposure or hybridization with higher concentration of primary antibody in some cell lines.
- Product Citations
-
- RRID
- AB_2832849 (BioLegend Cat. No. 690203) AB_2876743 (BioLegend Cat. No. 690204)
Antigen Details
- Structure
- 390 amino acids, predicted molecular weight of p38α is 41kD; molecular weight of other isoforms of p38α range from 29kDa to 35kD.
- Distribution
-
Cytoplasm, nucleaus
- Function
- Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK14 is one of the four p38 MAPKs which play an important role in the cascades of cellular responses evoked by extracellular stimuli such as proinflammatory cytokines or physical stress leading to direct activation of transcription factors.
- Interaction
- Component of a signaling complex containing at least AKAP13, PKN1, MAPK14, ZAK and MAP2K3. Binds to a kinase interaction motif within the protein tyrosine phosphatase, PTPRR. Interacts with SPAG9 and GADD45A. Interacts with CDC25B, CDC25C, DUSP1, DUSP10, DUSP16, NP60, SUPT20H and TAB1. Interacts with casein kinase II subunits CSNK2A1 and CSNK2B. Interacts with PPM1D. Interacts with CDK5RAP3; recruits PPM1D to MAPK14 and may regulate its dephosphorylation.
- Biology Area
- Cell Biology, Signal Transduction
- Molecular Family
- Phospho-Proteins, Protein Kinases/Phosphatase
- Antigen References
-
- Sosa MS, et al. 2011, Clin Cancer Res. 17:5850
- Igea A & Nebreda AR. 2015, Cancer Res. 75:3997
- Wagner EF & Nebreda AR. 2009, Nat Rev Cancer. 9:537
- Hui L, et al. 2007, Cell cycle. 6:2429
- Bulavin DV & Fornace AJ Jr. 2004, Adv Cancer Res. 92:95
- Gupta J, et al. 2014, Cancer cell. 25:484
- Otsuka M, et al. 2010, Gastroenterology. 138:1255
- Gene ID
- 1432 View all products for this Gene ID
- UniProt
- View information about p38 MAPK Phospho Thr180/Tyr182 on UniProt.org
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