Product Details
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Recombinant
- Host Species
- Mouse
- Immunogen
- Synthetic peptide corresponding to human p38 MAPK phosphorylated at threonine 180 and tryrosine 182
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
ICFC - Verified
- Recommended Usage
Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.5?- 1.0??g/mL. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125??g per million cells in 100 ?L volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
A16016A.Rec is a recombinant antibody derived from BioLegend clone A16016A (Cat. No. 690202).
This clone is predicted to recognize rat p38 MAPK phosphorylated at threonine 180 and tyrosine 182 due to complete sequence homology between the immunizing peptide and the rat p38 MAPK ortholog.- RRID
- AB_2910498 (BioLegend Cat. No. 690251) AB_2910498 (BioLegend Cat. No. 690252)
Antigen Details
- Structure
- 390 amino acids, predicted molecular weight of p38α is 41 kD; molecular weight of other isoforms of p38α range from 29 kD to 35 kD.
- Distribution
-
Cytoplasm and nucleus/Ubiquitously expressed
- Function
- Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK14 is one of the four p38 MAPKs which play an important role in the cascades of cellular responses evoked by extracellular stimuli such as proinflammatory cytokines or physical stress leading to direct activation of transcription factors.
- Interaction
- Component of a signaling complex containing at least AKAP13, PKN1, MAPK14, ZAK and MAP2K3. Binds to a kinase interaction motif within the protein tyrosine phosphatase, PTPRR. Interacts with SPAG9 and GADD45A. Interacts with CDC25B, CDC25C, DUSP1, DUSP10, DUSP16, NP60, SUPT20H and TAB1. Interacts with casein kinase II subunits CSNK2A1 and CSNK2B. Interacts with PPM1D. Interacts with CDK5RAP3; recruits PPM1D to MAPK14 and may regulate its dephosphorylation.
- Biology Area
- Cell Biology, Signal Transduction
- Molecular Family
- Phospho-Proteins, Protein Kinases/Phosphatase
- Antigen References
-
- Sosa MS, et al. 2011. Clin Cancer Res. 17:5850-7
- Igea A and Nebreda AR. 2015. Cancer Res. 75:3997-4002
- Wagner EF and Nebreda AR. 2009. Nat Rev Cancer. 9:537-49
- Hui L, et al. 2007. Cell Cycle. 6:2429-33
- Bulavin DV and Fornace AJ Jr. 2004. Adv Cancer Res. 92:95-118
- Gupta J, et al. 2014. Cancer Cell. 25:484-500
- Otsuka M, et al. 2010. Gastroenterology. 138:1255-65
- Gene ID
- 1432 View all products for this Gene ID
- UniProt
- View information about p38 MAPK Phospho on UniProt.org
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