Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant human CXCL9
- Formulation
- 0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is < 0.01 EU/?g of the protein (< 0.001 ng/?g of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.
- Application
-
Neut - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by neutralization of the chemotaxis induced by recombinant human CXCL9 (MIG) (Cat. No. 578106) at 0.2 ?g/mL on mouse Baf3-mCXCR3 transfectant chemoattractant cells. ND50 range: 0.4 - 3 ?g/mL. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
- AB_2894528 (BioLegend Cat. No. 943503) AB_2894528 (BioLegend Cat. No. 943504)
Antigen Details
- Structure
- Chemokine
- Distribution
-
Monocytes, macrophages, antigen-presenting cells (APC), eosinophils, endothelial cells, B cells, fibroblasts in inflammatory conditions
- Function
- Induces chemotaxis, promotes differentiation and multiplication of leukocytes, causes tissue extravasation, enhances CNS immunity; CXCL9/CXCR3 receptor regulates immune cell migration, differentiation, and activation.
- Interaction
- Activated T lymphocytes, CD4 Th1, CD8+, NK, NKT cells
- Ligand/Receptor
- CXCR3A, CXCR3B
- Cell Type
- Antigen-presenting cells, B cells, Endothelial cells, Eosinophils, Fibroblasts, Macrophages, Monocytes
- Biology Area
- Adaptive Immunity, Angiogenesis, Cardiovascular Biology, Immuno-Oncology, Immunology, Innate Immunity, Neuroscience, Signal Transduction, Stem Cells
- Molecular Family
- Cytokines/Chemokines
- Antigen References
-
1. Loetscher M, et al. 1996. J Exp Med. 184:963.
2. Lambeir AM, et al. 2001. J Biol Chem. 276:29839.
3. Lasagni L, et al. 2003. J Exp Med. 197:1537.
4. Whiting D, et al. 2004. J Immunol. 172:7417.
5. Proost P, et al. 2004. J Leuko Biol. 75:777.
6. Gorbachev AV, et al. 2007. J Immunol. 178: 2278.
7. Rosenblum JM, et al. 2010. J Immunol. 184:3450.
8. Crawford MA, et al. 2010. PLoS Pathog. 6:e1001199. - Gene ID
- 4283 View all products for this Gene ID
- UniProt
- View information about CXCL9 on UniProt.org
