Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human ZAP70 peptide phosphorylated at Tyr292. Complete Freund's adjuvant.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
ICFC - Quality tested
Direct ELISA - Reported in the literature, not verified in house - Recommended Usage
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
During development, reactivity was observed with mouse phospho-SYK.
- RRID
- AB_2686945 (BioLegend Cat. No. 693802)
Antigen Details
- Structure
- 619 amino acids with a predicted molecular weight of 70 kD.
- Distribution
-
Cytoplasm; Human T Cells, Mouse T cells and B cells.
- Function
- Required for the assembly of a Zap-70-containing signaling complex. Activation of the PLC-γ1-dependent and Ras-dependent signaling cascades. Associates with PLC-γ 1.
- Interaction
- NFAM1, Cbl, SLA and SLA2, CBLB, DEF6, FCRL3, VAV1, CD247/CD3Z.
- Cell Type
- B cells, T cells
- Biology Area
- Cell Biology, Immunology
- Molecular Family
- Phospho-Proteins, Protein Kinases/Phosphatase, TCRs
- Antigen References
-
1. Chan AC, et al. 1991. Proc. Natl. Acad. Sci. USA 88:9166.
2. Arpaia E, et al. 1994. Cell 76:947.
3. Chan AC, et al. 1994. Science 264:1599.
4. Negishi I, et al. 1995. Nature 376:435.
5. Wang H, et al. 2010. Cold Spring Harb. Perspect. Biol. 2:a002279.
6. Klammt C, et al. 2015. Nat. Immunol. 16:961.
7. Weiss A, et al. 1993. Cell 73:209.
8. Gong Q, et al. 2001. J. Exp. Med. 194:507.
9. Magnan A, et al. 2001. J. Exp. Med. 194:491. - Gene ID
- 7535 View all products for this Gene ID
- UniProt
- View information about ZAP70 Phospho Tyr292 on UniProt.org
